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Ki67 antibody [Ki-67]

Anti-Ki67 antibody [Ki-67] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00538

Cat. No. GTX00538

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 Ki-67

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, IHC

Reactivity

 Human, Bovine
Package
100 μg ($399)
See all Ki67 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
FACS Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

359 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Nuclei of the Hodgkin lymphoma cell line L428

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

KIA , MIB , MIB1 , MKI67 , PPP1R105 , marker of proliferation Ki67 , ki-67 , Ki67 , marker of proliferation Ki-67

Cellular Localization

Chromosome,Nucleus,Nucleolus

Background

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-Ki67 antibody [Ki-67] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00538

GTX00538 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX00538 Ki67 antibody [Ki-67].
Green : Primary antibody
Blue : DAPI

REFERENCE

Application Reference

Wang B et al. Int J Mol Sci 2024; 25 (3) The HIF-1α/EGF/EGFR Signaling Pathway Facilitates the Proliferation of Yak Alveolar Type II Epithelial Cells in Hypoxic Conditions.

Clone Reference

J La??ovi?ka et al. Hum Immunol 2016; 77 (12):1215-1222 Assessment of lymphocyte proliferation for diagnostic purpose: Comparison of CFSE staining, Ki-67 expression and 3H-thymidine incorporation
Application : ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399