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Ki67 antibody [MKI67/2462]

Anti-Ki67 antibody [MKI67/2462] used in Flow cytometry (FACS). GTX17971
Anti-Ki67 antibody [MKI67/2462] used in IHC (Paraffin sections) (IHC-P). GTX17971
Anti-Ki67 antibody [MKI67/2462] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17971
Anti-Ki67 antibody [MKI67/2462] used in IHC (Paraffin sections) (IHC-P). GTX17971
Anti-Ki67 antibody [MKI67/2462] used in Western Blot (WB). GTX17971
Anti-Ki67 antibody [MKI67/2462] used in Protein Array (Protein Array). GTX17971
Anti-Ki67 antibody [MKI67/2462] used in IHC (Paraffin sections) (IHC-P). GTX17971

Cat. No. GTX17971

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MKI67/2462

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, Protein Array

Reactivity

 Human
Package
100 μg ($529)
See all Ki67 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

359 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Ki67 protein fragment (around aa 2293-2478) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

KIA , MIB , MIB1 , MKI67 , PPP1R105 , marker of proliferation Ki67 , ki-67 , Ki67 , marker of proliferation Ki-67

Cellular Localization

Chromosome,Nucleus,Nucleolus

Background

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-Ki67 antibody [MKI67/2462] used in Flow cytometry (FACS). GTX17971

GTX17971 FACS Image

FACS analysis of HeLa cells using GTX17971 Ki67 antibody [MKI67/2462].
Blue : Primary antibody
Red : Isotype control

Anti-Ki67 antibody [MKI67/2462] used in IHC (Paraffin sections) (IHC-P). GTX17971

GTX17971 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX17971 Ki67 antibody [MKI67/2462].

Anti-Ki67 antibody [MKI67/2462] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17971

GTX17971 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX17971 Ki67 antibody [MKI67/2462].
Green : Primary antibody
Red : Phalloidin

Anti-Ki67 antibody [MKI67/2462] used in IHC (Paraffin sections) (IHC-P). GTX17971

GTX17971 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX17971 Ki67 antibody [MKI67/2462].

Anti-Ki67 antibody [MKI67/2462] used in Western Blot (WB). GTX17971

GTX17971 WB Image

WB analysis of Raji and Ramos cell lysates using GTX17971 Ki67 antibody [MKI67/2462].

Anti-Ki67 antibody [MKI67/2462] used in Protein Array (Protein Array). GTX17971

GTX17971 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Ki67-Monospecific Mouse Monoclonal Antibody (MKI67/2462). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Anti-Ki67 antibody [MKI67/2462] used in IHC (Paraffin sections) (IHC-P). GTX17971

GTX17971 IHC-P Image

IHC-P analysis of human tonsil-skin tissue using GTX17971 Ki67 antibody [MKI67/2462].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529