APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
1-2μg/ml for 30 minutes at RT |
1-2μg/10⁶ cells |
Assay dependent |
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa 2293-2478) of human Ki67 protein (exact sequence is proprietary)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
KIA , MIB , MIB1 , MKI67 , PPP1R105 , marker of proliferation Ki67 , ki-67 , Ki67 , marker of proliferation Ki-67
Cellular Localization
Chromosome,Nucleus,Nucleolus
Background
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
Database
Research Area
DATA IMAGES
|
GTX17972 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using Ki67 Mouse Monoclonal Antibody (MKI67/2465). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
|
|
GTX17972 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX17972 Ki67 antibody [MKI67/2465]. Green : Primary antibody Red : Phalloidin
|
|
GTX17972 FACS Image
FACS analysis of HeLa cells using GTX17972 Ki67 antibody [MKI67/2465]. Blue : Primary antibody Red : Isotype control
|
|
GTX17972 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX17972 Ki67 antibody [MKI67/2465].
|
REFERENCE
There are currently no references for Ki67 antibody [MKI67/2465] (GTX17972). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Ki67 antibody [MKI67/2465] (GTX17972). Be the first to share your experience with this product.