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Ki67 antibody [SP6]

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in Immunohistochemistry (IHC). GTX16667
Anti-Ki67 antibody [SP6] used in Immunohistochemistry (IHC). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in Immunohistochemistry (IHC). GTX16667

Cat. No. GTX16667

Host

Rabbit

Clonality

Monoclonal

Clone Name

SP6

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IHC

Reactivity

Human, Mouse, Rat, Chicken, Pig
Package
500 μl ($519)
PRODUCT

Summary

Ki67 antibody recognizes Ki67 antigen, a nuclear nonhistone protein with a predicted molecular weight of 359 kDa. Ki67 is present during the active phases of the cell cycle and is used clinically as a proliferation marker. Ki67 antibody is used extensively in immunohistochemistry to assess the grade or apparent aggressiveness of various malignancies.

APPLICATION

Application Note

IHC-P: 1:100-1:1000. Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. WB: 1/200. Predicted molecular weight: 359 kDa. Not tested in other applications

Calculated MW

359 kDa. ( Note )

Positive Control

Tonsil

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant, PBS, BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Antigen Species

Human

Purification

Tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_422351

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

marker of proliferation Ki-67 , KIA , MIB- , MIB-1 , PPP1R105

Cellular Localization

Nucleus , Nucleolus

Background

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus in human cervical metaplasia (left) and carcinoma (right) by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
Ki67 antibody [SP6] (GTX16667) diluted at 1:200.

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

The data was published in the journal Cell Death Dis in 2019. PMID: 31659152

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

The data was published in the journal Cell Death Dis in 2019. PMID: 31659152

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

The data was published in the journal Sci Rep in 2017. PMID: 28785010

Anti-Ki67 antibody [SP6] used in Immunohistochemistry (IHC). GTX16667

GTX16667 IHC Image

The data was published in the journal Nat Commun in 2016. PMID: 26965827

Anti-Ki67 antibody [SP6] used in Immunohistochemistry (IHC). GTX16667

GTX16667 IHC Image

The data was published in the journal Nat Commun in 2016. PMID: 26965827

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

The data was published in the journal PLoS One in 2015. PMID: 25747583

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

The data was published in the journal Front Oncol in 2020.PMID: 32426268

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

The data was published in the journal Nat Commun in 2016.PMID: 27187615

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

The data was published in the journal Nat Commun in 2016.PMID: 27187615

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

The data was published in the journal BMC Cancer in 2019.PMID: 30606143

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

The data was published in the journal Nat Commun in 2016.PMID: 27187615

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

The data was published in the 2021 in Fujita Med J. PMID: 35111543

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

The data was published in the 2021 in Fujita Med J. PMID: 35111543

Anti-Ki67 antibody [SP6] used in Immunohistochemistry (IHC). GTX16667

GTX16667 IHC Image

The data was published in the journal Nat Commun in 2016. PMID: 26965827

REFERENCE

REVIEW

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Application Reference
Sun Z et al. Life Sci 2020; 263:118672
Application : ICC/IF
Reactivity : Mouse
Wei J et al. J Cell Mol Med 2020; 24 (19):11366-11380
Application : IHC-P
Reactivity : Human
Gong L et al. Cancer Cell Int 2020; 20:194
Application : IHC-P
Reactivity : Mouse
Zhu Y et al. Oncogene 2019; (Epub)
Application : IHC-P
Reactivity : Human
Clone Reference
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519