GeneTex
United States (US)

Ki67 antibody [SP6]

GTX16667_20160307_IHC-P_18121410_764.jpg
GTX16667_821504294_20181130_IHC-P_1_19011519_290.jpg
GTX16667_821804180_20181130_IHC-P_19011519_988.jpg
GTX16667_821504294_20181130_IHC-P_19011519_685.jpg
GTX16667_821800349_20181130_IHC-P_1_19011519_828.jpg
GTX16667_821804180_20181130_IHC-P_1_19011519_743.jpg
GTX16667_821800349_20181130_IHC-P_19011519_807.jpg

Cat No. GTX16667

Host Rabbit
Clonality Monoclonal
Clone Name SP6
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IHC
Reactivity Human, Mouse, Rat, Rabbit
APPLICATION

Application Note

IHC-P: 1:100-1:1000. Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. WB: 1/200. Predicted molecular weight: 359 kDa. Not tested in other applications

Calculated MW

359 kDa. ( Note )

Positive Control

Tonsil
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Antigen Species

Human

Purification

Tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Marker Of Proliferation Ki-67,Kia,Mib-,Mib-1,Ppp1R105,Mki67

Cellular Localization

Nuclear

Background

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67-positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki67 index and histologically low-grade tumours is strong.

Database

Research Area

DATA IMAGES
GTX16667_20160307_IHC-P_18121410_764.jpg

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus in human cervical metaplasia (left) and carcinoma (right) by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
Ki67 antibody [SP6] (GTX16667) diluted at 1:200.

GTX16667_821504294_20181130_IHC-P_1_19011519_290.jpg

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX16667_821804180_20181130_IHC-P_19011519_988.jpg

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX16667_821504294_20181130_IHC-P_19011519_685.jpg

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX16667_821800349_20181130_IHC-P_1_19011519_828.jpg

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX16667_821804180_20181130_IHC-P_1_19011519_743.jpg

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX16667_821800349_20181130_IHC-P_19011519_807.jpg

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
Application Reference
Clone Reference
Package List Price ($)
$ 349