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Ki67 antibody [SP6]

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667
Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

Cat No. GTX16667

Host

Rabbit

Clonality

Monoclonal

Clone Name

SP6

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IHC

Reactivity

Human, Mouse, Rat, Chicken, Pig
Package
500 μl ($459)
PRODUCT

Summary

Ki67 antibody recognizes Ki67 antigen, a nuclear nonhistone protein with a predicted molecular weight of 359 kDa. Ki67 is present during the active phases of the cell cycle and is used clinically as a proliferation marker. Ki67 antibody is used extensively in immunohistochemistry to assess the grade or apparent aggressiveness of various malignancies.

APPLICATION

Application Note

IHC-P: 1:100-1:1000. Staining of formalin-fixed tissues is required by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. WB: 1/200. Predicted molecular weight: 359 kDa. Not tested in other applications

Calculated MW

359 kDa. ( Note )

Positive Control

Tonsil

PROPERTIES

Form

Liquid

Buffer

Tissue culture supernatant with PBS, BSA, and less than 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Antigen Species

Human

Purification

Tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_422351

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

marker of proliferation Ki-67 , KIA , MIB- , MIB-1 , PPP1R105

Cellular Localization

Nucleus , Nucleolus

Background

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
Ki67 stained by Ki67 antibody [SP6] (GTX16667) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ki67 antibody [SP6] used in IHC (Paraffin sections) (IHC-P). GTX16667

GTX16667 IHC-P Image

Ki67 antibody [SP6] detects Ki67 protein at nucleus in human cervical metaplasia (left) and carcinoma (right) by immunohistochemical analysis.
Sample: Paraffin-embedded human cervix.
Ki67 antibody [SP6] (GTX16667) diluted at 1:200.

REFERENCE

REVIEW

Ki67 antibody [SP6] Cat No. GTX16667

Rating ( Average 5 based on 7 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 4 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016.PMID: 26965827
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016.PMID: 26965827
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2016.PMID: 26965827
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2019.PMID: 31659152
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cell Death Dis in 2019.PMID: 31659152
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28785010
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25747583
Application Reference
Clone Reference
SDS
PBS.pdf
Sodium Azide.pdf