Application Note
For IHC-P: Use at a concentration of 1 0 - 2 0 μg / m l f o r 3 0 m i n a t R T. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Positive Control
Tonsil or Breast carcinoma
Form
Liquid
Buffer
10mM PBS, 0.2% BSA
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide from the human Ki-67 allantigen.
Purification
Protein A affinity purified
Conjugation
Unconjugated
RRID
AB_367700
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
marker of proliferation Ki-67 , KIA , MIB- , MIB-1 , PPP1R105
Cellular Localization
Nucleus , Nucleolus
Background
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
Database
Research Area