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Ki67 antibody

Anti-Ki67 antibody used in IHC (Paraffin sections) (IHC-P). GTX20833
Anti-Ki67 antibody used in Immunohistochemistry (IHC). GTX20833
Anti-Ki67 antibody used in Immunohistochemistry (IHC). GTX20833

Cat No. GTX20833

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

Human, Mouse, Rat
Package
500 μl ($319)

APPLICATION

Application Note

For IHC-P: Use at a concentration of 1 0 - 2 0 μg / m l f o r 3 0 m i n a t R T. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

359 kDa. ( Note )

Positive Control

Tonsil or Breast carcinoma

PROPERTIES

Form

Liquid

Buffer

10mM Phosphate-buffered saline containing 0.2% BSA and 0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide from the human Ki-67 allantigen.

Purification

Protein A affinity purified

Conjugation

Unconjugated

RRID

AB_367700

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

marker of proliferation Ki-67 , KIA , MIB- , MIB-1 , PPP1R105

Cellular Localization

Nucleus , Nucleolus

Background

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-Ki67 antibody used in IHC (Paraffin sections) (IHC-P). GTX20833

GTX20833 IHC-P Image

Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil using Ki-67 antibody. Signal is detected using ABC method with AEC chromogen

Anti-Ki67 antibody used in Immunohistochemistry (IHC). GTX20833

GTX20833 IHC Image

HT-29 xenograft tumor in mouse 20x pretreatment w/ citrate pH 6.0 using anti-Ki67 antibody GTX20833 at 5 ug/mL for 30 min at RT.

Anti-Ki67 antibody used in Immunohistochemistry (IHC). GTX20833

GTX20833 IHC Image

This picture was kindly supplied as part of the review submitted by Dr Robert O'Donogue. Tissue sections from mouse lung were stained with GTX20833 at a dilution of 1/50.

REFERENCE

REVIEW

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Application Reference
SDS
Sodium Azide.pdf