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Ki67 antibody

Anti-Ki67 antibody used in Immunohistochemistry (IHC). GTX20833
Anti-Ki67 antibody used in Immunohistochemistry (IHC). GTX20833
Anti-Ki67 antibody used in IHC (Paraffin sections) (IHC-P). GTX20833

Cat. No. GTX20833

Reference 10

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

 Human, Mouse, Rat
Package
500 μl ($399)
See all Ki67 products

APPLICATION

Application Note

For IHC-P: Use at a concentration of 1 0 - 2 0 μg / m l f o r 3 0 m i n a t R T. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

359 kDa. ( Note )

Positive Control

Tonsil or Breast carcinoma

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.2% BSA

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide from the human Ki-67 allantigen.

Purification

Protein A affinity purified

Conjugation

Unconjugated

RRID

AB_367700

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

marker of proliferation Ki-67 , KIA , MIB- , MIB-1 , PPP1R105

Cellular Localization

Nucleus , Nucleolus

Background

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-Ki67 antibody used in Immunohistochemistry (IHC). GTX20833

GTX20833 IHC Image

HT-29 xenograft tumor in mouse 20x pretreatment w/ citrate pH 6.0 using anti-Ki67 antibody GTX20833 at 5 ug/mL for 30 min at RT.

Anti-Ki67 antibody used in Immunohistochemistry (IHC). GTX20833

GTX20833 IHC Image

This picture was kindly supplied as part of the review submitted by Dr Robert O'Donogue. Tissue sections from mouse lung were stained with GTX20833 at a dilution of 1/50.

Anti-Ki67 antibody used in IHC (Paraffin sections) (IHC-P). GTX20833

GTX20833 IHC-P Image

Immunohistochemical analysis of formalin-fixed paraffin-embedded human tonsil using Ki-67 antibody. Signal is detected using ABC method with AEC chromogen

REFERENCE

Application Reference

P?rez-Mora S et al. Int J Mol Sci 2023; 24 (15) BFNB Enhances Hair Growth in C57BL/6 Mice through the Induction of EGF and FGF7 Factors and the PI3K-AKT-β-Catenin Pathway.
Cheng Wu et al. J Oncol 2022; 2022 : 1515416 Cholesterol Promotes Colorectal Cancer Growth by Activating the PI3K/AKT Pathway.
Reactivity : Mouse
Xu X et al. EMBO Mol Med 2021; 13 (7) : e14133 HSD17B7 gene in self-renewal and oncogenicity of keratinocytes from Black versus White populations.
Application : IHC-Fr
Reactivity : Mouse
Markezana A et al. Int J Hyperthermia 2021; 38 (1) : 263-272 Incomplete thermal ablation of tumors promotes increased tumorigenesis.
Application : IHC
Liu J et al. Mol Med Rep 2020; 22 (5) : 3667-3676 LncRNA HOTTIP promotes the proliferation and invasion of ovarian cancer cells by activating the MEK/ERK pathway.
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REVIEW

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Application Reference
P?rez-Mora S et al. Int J Mol Sci 2023; 24 (15) BFNB Enhances Hair Growth in C57BL/6 Mice through the Induction of EGF and FGF7 Factors and the PI3K-AKT-β-Catenin Pathway.
Cheng Wu et al. J Oncol 2022; 2022:1515416 Cholesterol Promotes Colorectal Cancer Growth by Activating the PI3K/AKT Pathway.
Reactivity : Mouse
Xu X et al. EMBO Mol Med 2021; 13 (7):e14133 HSD17B7 gene in self-renewal and oncogenicity of keratinocytes from Black versus White populations.
Application : IHC-Fr
Reactivity : Mouse
Markezana A et al. Int J Hyperthermia 2021; 38 (1):263-272 Incomplete thermal ablation of tumors promotes increased tumorigenesis.
Application : IHC
Liu J et al. Mol Med Rep 2020; 22 (5):3667-3676 LncRNA HOTTIP promotes the proliferation and invasion of ovarian cancer cells by activating the MEK/ERK pathway.
Nakazawa K et al. J Biol Chem 2020; 295 (44):15029-15044 Implications of RNG140 (caprin2)-mediated translational regulation in eye lens differentiation.
Application : WB
Reactivity : Human
Li M et al. Cell Death Dis 2020; 11 (1):31 The nuclear translocation of transketolase inhibits the farnesoid receptor expression by promoting the binding of HDAC3 to FXR promoter in hepatocellular carcinoma cell lines.
Application : WB
Reactivity : Mouse
Bordignon P et al. Cell Rep 2019; 28 (9):2358-2372.e6 Dualism of FGF and TGF-帣 Signaling in Heterogeneous Cancer-Associated Fibroblast Activation with ETV1 as a Critical Determinant.
Application : ICC/IF
Reactivity : Human
Chou CM et al. J Biomed Sci 2015; 22:102 Activation of MEK2 is sufficient to induce skin papilloma formation in transgenic zebrafish.
Application : IHC-P
Trazzi S et al. J Biol Chem 2010; 285 (13):10098-109 CB1 cannabinoid receptors increase neuronal precursor proliferation through AKT/glycogen synthase kinase-3beta/beta-catenin signaling.
Application : ICC/IF
Reactivity : Mouse
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399