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Ki67 (phospho Thr1335) antibody

Orthogonal
Anti-Ki67 (phospho Thr1335) antibody used in IHC (Paraffin sections) (IHC-P). GTX03435
Anti-Ki67 (phospho Thr1335) antibody used in IHC (Paraffin sections) (IHC-P). GTX03435
Anti-Ki67 (phospho Thr1335) antibody used in IHC (Paraffin sections) (IHC-P). GTX03435
Anti-Ki67 (phospho Thr1335) antibody used in IHC (Paraffin sections) (IHC-P). GTX03435
Anti-Ki67 (phospho Thr1335) antibody used in IHC (Paraffin sections) (IHC-P). GTX03435
Anti-Ki67 (phospho Thr1335) antibody used in Western Blot (WB). GTX03435

Cat. No. GTX03435

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($449)
See all Ki67 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

359 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human Ki67 around the phosphorylation site of Thr1335.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

marker of proliferation Ki-67 , KIA , MIB- , MIB-1 , PPP1R105

Cellular Localization

Nucleus , Nucleolus

Background

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Database

Research Area

DATA IMAGES

Anti-Ki67 (phospho Thr1335) antibody used in IHC (Paraffin sections) (IHC-P). GTX03435

GTX03435 IHC-P Image

IHC-P analysis of rat testis tissue using GTX03435 Ki67 (phospho Thr1335) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Ki67 (phospho Thr1335) antibody used in IHC (Paraffin sections) (IHC-P). GTX03435

GTX03435 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX03435 Ki67 (phospho Thr1335) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Ki67 (phospho Thr1335) antibody used in IHC (Paraffin sections) (IHC-P). GTX03435

GTX03435 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX03435 Ki67 (phospho Thr1335) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Ki67 (phospho Thr1335) antibody used in IHC (Paraffin sections) (IHC-P). GTX03435

GTX03435 IHC-P Image

IHC-P analysis of human ovarian cancer tissue using GTX03435 Ki67 (phospho Thr1335) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Ki67 (phospho Thr1335) antibody used in IHC (Paraffin sections) (IHC-P). GTX03435

GTX03435 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX03435 Ki67 (phospho Thr1335) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Ki67 (phospho Thr1335) antibody used in Western Blot (WB). GTX03435

GTX03435 WB Image

Orthogonal

WB analysis of various sample lysates using GTX03435 Ki67 (phospho Thr1335) antibody.
Lane 1 : K562 cells (heat shock treatment), blocked with antigen-specific peptides
Lane 2 : K562 cells (heat shock treatment)
Lane 3 : RAW264.7 cells (LPS treatment, 4hr).

REFERENCE

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REVIEW

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Package List Price ($)
$ 449