GeneTex
United States (US)

Kiss1 antibody

GTX130503_42179_20150813_WB_M_testis_18121410_894.jpg
GTX130503_42179_20170216_WB_18121410_250.jpg
GTX130503_42179_20171222_WB_R_testis_18121410_659.jpg

Cat No. GTX130503

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929 , mouse testis , rat testis
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center of human Kiss1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Kiss-1 Metastasis Suppressor,Hh13,Kiss-1,Kiss1

Cellular Localization

Secreted

Background

This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may function to inhibit chemotaxis and invasion, attenuating metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX130503_42179_20150813_WB_M_testis_18121410_894.jpg

GTX130503 WB Image

KISS1 antibody detects KISS1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with KISS1 antibody (GTX130503) diluted at 1:1000.

GTX130503_42179_20170216_WB_18121410_250.jpg

GTX130503 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with KISS1 antibody (GTX130503) diluted at 1:1000.

GTX130503_42179_20171222_WB_R_testis_18121410_659.jpg

GTX130503 WB Image

Rat tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Kiss1 antibody (GTX130503) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169