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Ku70 antibody [1.5]

Anti-Ku70 antibody [1.5] used in Western Blot (WB). GTX70271
Anti-Ku70 antibody [1.5] used in Western Blot (WB). GTX70271

Cat. No. GTX70271

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1.5

Isotype

 IgG2b

Application

 WB

Reactivity

 Human
Package
100 μl ($399)
See all Ku70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Raji

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant antigen containing the amino terminal portion of human Ku70 protein

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_372048

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

X-ray repair cross complementing 6 , CTC75 , CTCBF , G22P1 , KU70 , ML8 , TLAA

Cellular Localization

Nucleus

Background

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ku70 antibody [1.5] used in Western Blot (WB). GTX70271

GTX70271 WB Image

Detection of human Ku70 protein in HeLa whole cell lysate using GeneTex Ku70 1.5 monoclonal antibody GTX70271.

Anti-Ku70 antibody [1.5] used in Western Blot (WB). GTX70271

GTX70271 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku70 antibody [1.5] (GTX70271) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Jalili P et al. Nat Commun 2020; 11 (1) : 2971 Quantification of ongoing APOBEC3A activity in tumor cells by monitoring RNA editing at hotspots.
Reactivity : Human
Nguyen HD et al. Cancer Res 2018; (Epub) Spliceosome Mutations Induce R loop-Associated Sensitivity to ATR Inhibition in Myelodysplastic Syndrome.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations