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Ku70 antibody [1.5]

Anti-Ku70 antibody [1.5] used in Western Blot (WB). GTX70271
Anti-Ku70 antibody [1.5] used in Western Blot (WB). GTX70271

Cat No. GTX70271

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1.5

Isotype

 IgG2b

Application

 WB

Reactivity

 Human
Package
100 μl ($329)
See all Ku70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Raji

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant antigen containing the amino terminal portion of human Ku70 protein

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-Ray Repair Cross Complementing 6 , Ctc75 , Ctcbf , G22P1 , Ku70 , Ml8 , Tlaa , Xrcc6

Background

Originally identified as an autoantigen, the Ku protein are nuclear proteins found in eukaryotes. They exist as a heterodimer consisiting of Ku 70 and Ku 80, migrating at approximately 70 and 80 kDa respectively. They bind to DNA ends at double strand breaks. The Ku proteins interact with the DNA-dependent protein kinase (DNA-PK) and participate in the targeting of DNA-PK to DNA. The Ku proteins may participate in numerous cellular processes, including DNA repair and replication, cell signalling, cell proliferation, transcriptional activation and apoptosis.

Database

Research Area

DATA IMAGES

Anti-Ku70 antibody [1.5] used in Western Blot (WB). GTX70271

GTX70271 WB Image

Detection of human Ku70 protein in HeLa whole cell lysate using GeneTex Ku70 1.5 monoclonal antibody GTX70271.

Anti-Ku70 antibody [1.5] used in Western Blot (WB). GTX70271

GTX70271 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku70 antibody [1.5] (GTX70271) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Jalili P et al. Nat Commun 2020; 11 (1) : 2971 Quantification of ongoing APOBEC3A activity in tumor cells by monitoring RNA editing at hotspots.
Reactivity : Human
Nguyen HD et al. Cancer Res 2018; (Epub) Spliceosome Mutations Induce R loop-Associated Sensitivity to ATR Inhibition in Myelodysplastic Syndrome.
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329