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Ku70 antibody [1.5]

Anti-Ku70 antibody [1.5] used in Western Blot (WB). GTX70271
Anti-Ku70 antibody [1.5] used in Western Blot (WB). GTX70271

Cat No. GTX70271

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1.5

Isotype

 IgG2b

Application

 WB

Reactivity

 Human
Package
100 μl ($329)
See all Ku70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , Raji

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant antigen containing the amino terminal portion of human Ku70 protein

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_372048

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-ray repair cross complementing 6 , CTC75 , CTCBF , G22P1 , KU70 , ML8 , TLAA

Cellular Localization

Nucleus

Background

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ku70 antibody [1.5] used in Western Blot (WB). GTX70271

GTX70271 WB Image

Detection of human Ku70 protein in HeLa whole cell lysate using GeneTex Ku70 1.5 monoclonal antibody GTX70271.

Anti-Ku70 antibody [1.5] used in Western Blot (WB). GTX70271

GTX70271 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku70 antibody [1.5] (GTX70271) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Jalili P et al. Nat Commun 2020; 11 (1) : 2971 Quantification of ongoing APOBEC3A activity in tumor cells by monitoring RNA editing at hotspots.
Reactivity : Human
Nguyen HD et al. Cancer Res 2018; (Epub) Spliceosome Mutations Induce R loop-Associated Sensitivity to ATR Inhibition in Myelodysplastic Syndrome.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
Custom Conjugations