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Ku70 antibody [529]

Anti-Ku70 antibody [529] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX77607
Anti-Ku70 antibody [529] used in Western Blot (WB). GTX77607
Anti-Ku70 antibody [529] used in Western Blot (WB). GTX77607
Anti-Ku70 antibody [529] used in Western Blot (WB). GTX77607

Cat No. GTX77607

Host

Mouse

Clonality

Monoclonal

Clone Name

529

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:5000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant antigen containing the amino terminal portion of human Ku70 protein

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_424561

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-ray repair cross complementing 6 , CTC75 , CTCBF , G22P1 , KU70 , ML8 , TLAA

Cellular Localization

Nucleus

Background

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ku70 antibody [529] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX77607

GTX77607 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Ku70(GTX77607) antibody (green) at 1:2000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2000.

Anti-Ku70 antibody [529] used in Western Blot (WB). GTX77607

GTX77607 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
7.5% SDS PAGE
GTX77607 diluted at 1:5000

Anti-Ku70 antibody [529] used in Western Blot (WB). GTX77607

GTX77607 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
7.5% SDS PAGE
GTX77607 diluted at 1:1000

Anti-Ku70 antibody [529] used in Western Blot (WB). GTX77607

GTX77607 WB Image

Detection of human Ku70 protein in HeLa whole cell lysate using GeneTex Ku70 529 monoclonal antibody GTX77607.

REFERENCE

REVIEW

Ku70 antibody [529] Cat No. GTX77607

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2015. PMID: 26215093
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019. PMID: 31164689
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019. PMID: 31164689
SDS
PBS.pdf
Package List Price ($)
$ 379
Custom Conjugations