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Ku70 antibody [N1N3]

Anti-Ku70 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101848
Anti-Ku70 antibody [N1N3] used in Western Blot (WB). GTX101848
Anti-Ku70 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101848

Cat No. GTX101848

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Ku70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-ray repair cross complementing 6 , CTC75 , CTCBF , G22P1 , KU70 , ML8 , TLAA

Cellular Localization

Nucleus

Background

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Ku70 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101848

GTX101848 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Ku70(GTX101848) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Ku70 antibody [N1N3] used in Western Blot (WB). GTX101848

GTX101848 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX101848 diluted at 1:1000

Anti-Ku70 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101848

GTX101848 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Ku70(GTX101848) antibody at 1:500 dilution.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169