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Ku70 antibody [N3H10]

Cat No. GTX23114

Host

Mouse

Clonality

Monoclonal

Clone Name

N3H10

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Hamster, Monkey, Xenopus laevis
Package
500 μl ($319)

APPLICATION

Application Note

Immunofluorescence: assay dependent. Immunoprecipitation (with protein A): use at a concentration of 2μg/mg of protein lysate (native and denatured). Western blotting: use at a concentration of 0.25 - 0.5μg/ml for 2hrs at RT. Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 1 - 2μg/ml for 30 minute at RT. Optimal dilutions / concentrations should be determined by the end user. Staining of formalin-fixed tissues is improved by boiling tissue sections in 10mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 min prior to immunostaining. By Western blot, this antibody detects a band of 70 kDa, which corresponds to the predicted molecular weight of Ku 70.

Calculated MW

70 kDa. ( Note )

Positive Control

BT474, HEP-G-2 or HeLa cells. Tonsil tissue sections.

PROPERTIES

Form

Liquid

Buffer

10mM PBS pH7.4, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human placental extract designated as PSE1-PL.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_367103

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-ray repair cross complementing 6 , CTC75 , CTCBF , G22P1 , KU70 , ML8 , TLAA

Cellular Localization

Nucleus

Background

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

REVIEW

Ku70 antibody [N3H10] Cat No. GTX23114

Rating ( Average 5 based on 5 users reviews)
Application
Western Blot(WB) ( Average 5 based on 4 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oxid Med Cell Longev in 2015. PMID: 26273418
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Genet in 2016. PMID: 27437695
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Genet in 2016. PMID: 27437695
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal F1000Res in 2014. PMID: 25177484
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019. PMID: 31164689
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319