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Ku70 antibody [N3H10]

Orthogonal
Anti-Ku70 antibody [N3H10] used in Western Blot (WB). GTX23114
Anti-Ku70 antibody [N3H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23114
Anti-Ku70 antibody [N3H10] used in Western Blot (WB). GTX23114
Anti-Ku70 antibody [N3H10] used in Western Blot (WB). GTX23114
Anti-Ku70 antibody [N3H10] used in Western Blot (WB). GTX23114

Cat. No. GTX23114

Reference 9

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 N3H10

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Hamster, Monkey, Xenopus laevis
Package
500 μl ($399)
See all Ku70 products

APPLICATION

Application Note

Immunofluorescence: assay dependent. Immunoprecipitation (with protein A): use at a concentration of 2μg/mg of protein lysate (native and denatured). Western blotting: use at a concentration of 0.25 - 0.5μg/ml for 2hrs at RT. Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 1 - 2μg/ml for 30 minute at RT. Optimal dilutions / concentrations should be determined by the end user. Staining of formalin-fixed tissues is improved by boiling tissue sections in 10mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 min prior to immunostaining. By Western blot, this antibody detects a band of 70 kDa, which corresponds to the predicted molecular weight of Ku 70.

Calculated MW

70 kDa. ( Note )

Positive Control

BT474, HEP-G-2 or HeLa cells. Tonsil tissue sections.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human placental extract designated as PSE1-PL.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_367103

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

X-ray repair cross complementing 6 , CTC75 , CTCBF , G22P1 , KU70 , ML8 , TLAA

Cellular Localization

Nucleus

Background

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ku70 antibody [N3H10] used in Western Blot (WB). GTX23114

GTX23114 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2019. PMID: 31164689

Anti-Ku70 antibody [N3H10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX23114

GTX23114 ICC/IF Image

The data was published in the journal PLoS Genet in 2016. PMID: 27437695

Anti-Ku70 antibody [N3H10] used in Western Blot (WB). GTX23114

GTX23114 WB Image

The data was published in the journal PLoS Genet in 2016. PMID: 27437695

Anti-Ku70 antibody [N3H10] used in Western Blot (WB). GTX23114

GTX23114 WB Image

The data was published in the journal F1000Res in 2014. PMID: 25177484

Anti-Ku70 antibody [N3H10] used in Western Blot (WB). GTX23114

GTX23114 WB Image

The data was published in the journal Nucleic Acids Res in 2014.PMID: 24589584

REFERENCE

Application Reference

Lin RC et al. Oncol Rep 2023; 50 (3) Polo?like kinase 1 selective inhibitor BI2536 (dihydropteridinone) disrupts centrosome homeostasis via ATM?ERK cascade in adrenocortical carcinoma.
Xiao H et al. Cells 2022; 11 (13) Increased Resection at DSBs in G(2)-Phase Is a Unique Phenotype Associated with DNA-PKcs Defects That Is Not Shared by Other Factors of c-NHEJ.
Application : WB
Reactivity : Human
Soni A et al. DNA Repair (Amst) 2020; 89 : 102828 Chromosome breaks generated by low doses of ionizing radiation in G2-phase are processed exclusively by gene conversion.
Application : WB
Reactivity : Hamster
Mladenov E et al. Sci Rep 2019; 9 (1) : 8255 Radiation-dose-dependent functional synergisms between ATM
Patidar PL et al. Nucleic Acids Res 2016; 44 (4) : 1718-31 The Kub5-Hera/RPRD1B interactome: a novel role in preserving genetic stability by regulating DNA mismatch repair.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Lin RC et al. Oncol Rep 2023; 50 (3) Polo?like kinase 1 selective inhibitor BI2536 (dihydropteridinone) disrupts centrosome homeostasis via ATM?ERK cascade in adrenocortical carcinoma.
Xiao H et al. Cells 2022; 11 (13) Increased Resection at DSBs in G(2)-Phase Is a Unique Phenotype Associated with DNA-PKcs Defects That Is Not Shared by Other Factors of c-NHEJ.
Application : WB
Reactivity : Human
Soni A et al. DNA Repair (Amst) 2020; 89:102828 Chromosome breaks generated by low doses of ionizing radiation in G2-phase are processed exclusively by gene conversion.
Application : WB
Reactivity : Hamster
Mladenov E et al. Sci Rep 2019; 9 (1):8255 Radiation-dose-dependent functional synergisms between ATM
Patidar PL et al. Nucleic Acids Res 2016; 44 (4):1718-31 The Kub5-Hera/RPRD1B interactome: a novel role in preserving genetic stability by regulating DNA mismatch repair.
Application : WB
Reactivity : Human
Morales JC et al. PLoS Genet 2016; 12 (7):e1006107 XRN2 Links Transcription Termination to DNA Damage and Replication Stress.
Application : WB
Reactivity : Human
Yang CC et al. F1000Research 2014; Systems analysis of the prostate tumor suppressor NKX3. 1 supports roles in DNA repair and luminal cell differentiation
Application : WB
Reactivity : Human
Morales JC et al. Nucleic Acids Res 2014; Kub5-Hera, the human Rtt103 homolog, plays dual functional roles in transcription termination and DNA repair.
Application : WB
Reactivity : Mouse
You BJ et al. Food Chem Toxicol 2014; 65:205-12 Non-homologous end joining pathway is the major route of protection against 4β-hydroxywithanolide E-induced DNA damage in MCF-7 cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399