Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:500-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.25mg/ml(Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Ku70. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
CTC75 antibody, CTCBF antibody, G22P1 antibody, KU70 antibody, ML8 antibody, TLAA antibody, XRCC6 antibody, CTC box-binding factor 75 kDa subunit antibody, ATP-dependent DNA helicase II, 70 kDa subunit antibody, ATP-dependent DNA helicase II 70 kDa subunit antibody, X-ray repair cross-complementing protein 6 antibody, ATP-dependent DNA helicase 2 subunit 1 antibody, lupus Ku autoantigen protein p70 antibody, thyroid autoantigen 70kD (Ku antigen) antibody, 5'-deoxyribose-5-phosphate lyase Ku70 antibody, Ku autoantigen, 70kDa antibody, DNA repair protein XRCC6 antibody, thyroid-lupus autoantigen p70 antibody, thyroid autoantigen 70kDa (Ku antigen) antibody, CTC box binding factor 75 kDa subunit antibody, 70 kDa subunit of Ku antigen antibody, 5'-dRP lyase Ku70 antibody, Ku autoantigen p70 subunit antibody, X-ray repair complementing defective repair in Chinese hamster cells 6 antibody
Cellular Localization
Nucleus
Background
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq]
Database
