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Ku70 antibody

Anti-Ku70 antibody used in Immunoprecipitation (IP). GTX101820
Anti-Ku70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101820
Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101820
Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101820
Anti-Ku70 antibody used in Western Blot (WB). GTX101820

Cat No. GTX101820

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, PLA

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
PLA Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Ku70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241460

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-ray repair cross complementing 6 , CTC75 , CTCBF , G22P1 , KU70 , ML8 , TLAA

Cellular Localization

Nucleus

Background

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Ku70 antibody used in Immunoprecipitation (IP). GTX101820

GTX101820 IP Image

Ku70 antibody immunoprecipitates Ku70 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Ku70 protein by 2.5 μg of Ku70 antibody (GTX101820) 7.5% SDS-PAGE The immunoprecipitated Ku70 protein was detected by Ku70 antibody (GTX101820) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Ku70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101820

GTX101820 ICC/IF Image

Ku70 antibody detects Ku70 protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ku70 protein stained by Ku70 antibody (GTX101820) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101820

GTX101820 IHC-P Image

Immunohistochemical analysis of paraffin-embedded D54 xenograft, using Ku70(GTX101820) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101820

GTX101820 IHC-P Image

Ku70 antibody detects Ku70 protein at nucleus in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
Ku70 antibody (GTX101820) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ku70 antibody used in Western Blot (WB). GTX101820

GTX101820 WB Image

Ku70 antibody detects Ku70 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku70 antibody (GTX101820) diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Ku70 antibody Cat No. GTX101820

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Cell Commun Signal in 2014. PMID: 25421435
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169