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Ku70 antibody

Anti-Ku70 antibody used in Immunoprecipitation (IP). GTX101820
Anti-Ku70 antibody used in Western Blot (WB). GTX101820
Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101820
Anti-Ku70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101820
Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101820
Anti-Ku70 antibody used in Western Blot (WB). GTX101820

Cat. No. GTX101820

Reference 18

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, PLA

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Ku70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
PLA Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Ku70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241460

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

X-ray repair cross complementing 6 , CTC75 , CTCBF , G22P1 , KU70 , ML8 , TLAA

Cellular Localization

Nucleus

Background

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Ku70 antibody used in Immunoprecipitation (IP). GTX101820

GTX101820 IP Image

Ku70 antibody immunoprecipitates Ku70 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Ku70 protein by 2.5 μg of Ku70 antibody (GTX101820) 7.5% SDS-PAGE The immunoprecipitated Ku70 protein was detected by Ku70 antibody (GTX101820) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Ku70 antibody used in Western Blot (WB). GTX101820

GTX101820 WB Image

Ku70 antibody detects Ku70 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku70 antibody (GTX101820) diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101820

GTX101820 IHC-P Image

Ku70 antibody detects Ku70 protein at nucleus in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
Ku70 antibody (GTX101820) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ku70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101820

GTX101820 ICC/IF Image

Ku70 antibody detects Ku70 protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ku70 protein stained by Ku70 antibody (GTX101820) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101820

GTX101820 IHC-P Image

Immunohistochemical analysis of paraffin-embedded D54 xenograft, using Ku70(GTX101820) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Ku70 antibody used in Western Blot (WB). GTX101820

GTX101820 WB Image

The data was published in the journal Cell Commun Signal in 2014. PMID: 25421435

REFERENCE

Application Reference

Islam A et al. Nucleic Acids Res 2024; 52 (5) : 2416-2433 Site-specific acetylation of polynucleotide kinase 3'-phosphatase regulates its distinct role in DNA repair pathways.
Chakraborty A et al. bioRxiv 2023; A glycolytic metabolite restores DNA repair activity of polynucleotide kinase 3'-phosphatase in polyglutamine (PolyQ) diseases.
Mori S et al. J Virol 2023; 97 (8) : e0081523 Nuclear proinflammatory cytokine S100A9 enhances expression of human papillomavirus oncogenes via transcription factor TEAD1.
Chakraborty A et al. J Biol Chem 2023; 299 (3) : 102991 Human DNA polymerase η promotes RNA-templated error-free repair of DNA double-strand breaks.
Rowley PA et al. G3 (Bethesda) 2021; 11 (8) Nuku, a family of primate retrocopies derived from KU70.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Islam A et al. Nucleic Acids Res 2024; 52 (5):2416-2433 Site-specific acetylation of polynucleotide kinase 3'-phosphatase regulates its distinct role in DNA repair pathways.
Chakraborty A et al. bioRxiv 2023; A glycolytic metabolite restores DNA repair activity of polynucleotide kinase 3'-phosphatase in polyglutamine (PolyQ) diseases.
Mori S et al. J Virol 2023; 97 (8):e0081523 Nuclear proinflammatory cytokine S100A9 enhances expression of human papillomavirus oncogenes via transcription factor TEAD1.
Chakraborty A et al. J Biol Chem 2023; 299 (3):102991 Human DNA polymerase η promotes RNA-templated error-free repair of DNA double-strand breaks.
Rowley PA et al. G3 (Bethesda) 2021; 11 (8) Nuku, a family of primate retrocopies derived from KU70.
Application : WB
Reactivity : Human
Ma M et al. J Exp Med 2021; 218 (2) Sustained androgen receptor signaling is a determinant of melanoma cell growth potential and tumorigenesis.
Chen TY et al. Cell Death Differ 2021; (Epub) Genotoxic stress-activated DNA-PK-p53 cascade and autophagy cooperatively induce ciliogenesis to maintain the DNA damage response.
Application : WB
Reactivity : Human
Chen TY et al. J Cell Physiol 2020; (Epub) Septin 7 is a centrosomal protein that ensures S phase entry and microtubule nucleation by maintaining the abundance of p150glued.
Wang CY et al. Drug Des Devel Ther 2020; 14:2987-3000 Cordycepin Inhibits Human Gestational Choriocarcinoma Cell Growth by Disrupting Centrosome Homeostasis.
Application : WB
Reactivity : Human
Bottoni G et al. Nat Commun 2019; 10 (1):3884 CSL controls telomere maintenance and genome stability in human dermal fibroblasts.
Application : WB, PLA
Chen MK et al. J Biol Chem 2019; 294 (21):8516-8528 H2O2 induces nuclear transport of the receptor tyrosine kinase c-MET in breast cancer cells via a membrane-bound retrograde trafficking mechanism.
Application : WB
Reactivity : Human
Chen TY et al. J Cell Physiol 2018; (Epub) Chloroquine inhibits human retina pigmented epithelial cell growth and microtubule nucleation by downregulating p150glued.
Application : WB
Reactivity : Human
Lien WC et al. J Cell Biochem 2017; (Epub) 7-hydroxy-staurosporine, UCN-01, induces DNA damage response and autophagy in human osteosarcoma U2-OS cells.
Application : WB
Reactivity : Human
Chen X et al. Carcinogenesis 2017;:43110 Dicer regulates non-homologous end joining and is associated with chemosensitivity in colon cancer patients.
Application : WB
Reactivity : Human
Zimmerer RM et al. Microvasc Res 2017; 112:101-108 CD24+ tumor-initiating cells from oral squamous cell carcinoma induce initial angiogenesis in vivo.
Application : IHC-P
Reactivity : Human
Tan W et al. Cancer Med 2017; (Epub) Overexpression of TNKS1BP1 in lung cancers and its involvement in homologous recombination pathway of DNA double-strand breaks.
Application : WB
Reactivity : Human
Chakraborty A et al. Nat Commun 2016; 7:13049 Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes.
Application : WB
Reactivity : Human
Wang CY et al. Cell Commun Signal 2014; 12:55 NR5A1 prevents centriole splitting by inhibiting centrosomal DNA-PK activation and β-catenin accumulation.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169