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Ku70 antibody

Anti-Ku70 antibody used in Immunoprecipitation (IP). GTX101820
Anti-Ku70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101820
Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101820
Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101820
Anti-Ku70 antibody used in Western Blot (WB). GTX101820

Cat No. GTX101820

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, PLA

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all Ku70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
PLA Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Ku70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-ray repair cross complementing 6 , CTC75 , CTCBF , G22P1 , KU70 , ML8 , TLAA

Cellular Localization

Nucleus

Background

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Ku70 antibody used in Immunoprecipitation (IP). GTX101820

GTX101820 IP Image

Ku70 antibody immunoprecipitates Ku70 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Ku70 protein by 2.5 μg of Ku70 antibody (GTX101820) 7.5% SDS-PAGE The immunoprecipitated Ku70 protein was detected by Ku70 antibody (GTX101820) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Ku70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101820

GTX101820 ICC/IF Image

Ku70 antibody detects Ku70 protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ku70 protein stained by Ku70 antibody (GTX101820) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101820

GTX101820 IHC-P Image

Immunohistochemical analysis of paraffin-embedded D54 xenograft, using Ku70(GTX101820) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX101820

GTX101820 IHC-P Image

Ku70 antibody detects Ku70 protein at nucleus in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
Ku70 antibody (GTX101820) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Ku70 antibody used in Western Blot (WB). GTX101820

GTX101820 WB Image

Ku70 antibody detects Ku70 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku70 antibody (GTX101820) diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Bottoni G et al. Nat Commun 2019; 10 (1) : 3884 CSL controls telomere maintenance and genome stability in human dermal fibroblasts.
Application : WB, PLA
Chen MK et al. J Biol Chem 2019; 294 (21) : 8516-8528 H2O2 induces nuclear transport of the receptor tyrosine kinase c-MET in breast cancer cells via a membrane-bound retrograde trafficking mechanism.
Application : WB
Reactivity : Human
Chen TY et al. J Cell Physiol 2018; (Epub) Chloroquine inhibits human retina pigmented epithelial cell growth and microtubule nucleation by downregulating p150glued.
Application : WB
Reactivity : Human
Lien WC et al. J Cell Biochem 2017; (Epub) 7-hydroxy-staurosporine, UCN-01, induces DNA damage response and autophagy in human osteosarcoma U2-OS cells.
Application : WB
Reactivity : Human
Chen X et al. Carcinogenesis 2017; : 43110 Dicer regulates non-homologous end joining and is associated with chemosensitivity in colon cancer patients.
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Bottoni G et al. Nat Commun 2019; 10 (1):3884 CSL controls telomere maintenance and genome stability in human dermal fibroblasts.
Application : WB, PLA
Chen MK et al. J Biol Chem 2019; 294 (21):8516-8528 H2O2 induces nuclear transport of the receptor tyrosine kinase c-MET in breast cancer cells via a membrane-bound retrograde trafficking mechanism.
Application : WB
Reactivity : Human
Chen TY et al. J Cell Physiol 2018; (Epub) Chloroquine inhibits human retina pigmented epithelial cell growth and microtubule nucleation by downregulating p150glued.
Application : WB
Reactivity : Human
Lien WC et al. J Cell Biochem 2017; (Epub) 7-hydroxy-staurosporine, UCN-01, induces DNA damage response and autophagy in human osteosarcoma U2-OS cells.
Application : WB
Reactivity : Human
Chen X et al. Carcinogenesis 2017;:43110 Dicer regulates non-homologous end joining and is associated with chemosensitivity in colon cancer patients.
Application : WB
Reactivity : Human
Zimmerer RM et al. Microvasc Res 2017; 112:101-108 CD24+ tumor-initiating cells from oral squamous cell carcinoma induce initial angiogenesis in vivo.
Application : IHC-P
Reactivity : Human
Tan W et al. Cancer Med 2017; (Epub) Overexpression of TNKS1BP1 in lung cancers and its involvement in homologous recombination pathway of DNA double-strand breaks.
Application : WB
Reactivity : Human
Chakraborty A et al. Nat Commun 2016; 7:13049 Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes.
Application : WB
Reactivity : Human
Wang CY et al. Cell Commun Signal 2014; 12:55 NR5A1 prevents centriole splitting by inhibiting centrosomal DNA-PK activation and β-catenin accumulation.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169