GeneTex
United States (US)

Ku70 antibody

GTX101820_39577_IP_19011506_771.jpg
GTX101820_41990_20150525_IFA_19011506_122.jpg
GTX101820_39577_IHC_19011506_876.jpg
GTX101820_41990_20150518_IHC-P_19011506_703.jpg
GTX101820_41892_20141121_WB_19011506_341.jpg

Cat No. GTX101820

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Ku70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CTC75 antibody, CTCBF antibody, G22P1 antibody, KU70 antibody, ML8 antibody, TLAA antibody, XRCC6 antibody, CTC box-binding factor 75 kDa subunit antibody, ATP-dependent DNA helicase II, 70 kDa subunit antibody, ATP-dependent DNA helicase II 70 kDa subunit antibody, X-ray repair cross-complementing protein 6 antibody, ATP-dependent DNA helicase 2 subunit 1 antibody, lupus Ku autoantigen protein p70 antibody, thyroid autoantigen 70kD (Ku antigen) antibody, 5'-deoxyribose-5-phosphate lyase Ku70 antibody, Ku autoantigen, 70kDa antibody, DNA repair protein XRCC6 antibody, thyroid-lupus autoantigen p70 antibody, thyroid autoantigen 70kDa (Ku antigen) antibody, CTC box binding factor 75 kDa subunit antibody, 70 kDa subunit of Ku antigen antibody, 5'-dRP lyase Ku70 antibody, Ku autoantigen p70 subunit antibody, X-ray repair complementing defective repair in Chinese hamster cells 6 antibody

Cellular Localization

Nucleus

Background

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq]

Database

DATA IMAGES
GTX101820_39577_IP_19011506_771.jpg

GTX101820 IP Image

Ku70 antibody immunoprecipitates Ku70 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of Ku70 protein by 2.5 μg of Ku70 antibody (GTX101820) 7.5% SDS-PAGE The immunoprecipitated Ku70 protein was detected by Ku70 antibody (GTX101820) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX101820_41990_20150525_IFA_19011506_122.jpg

GTX101820 ICC/IF Image

Ku70 antibody detects Ku70 protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ku70 protein stained by Ku70 antibody (GTX101820) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

GTX101820_39577_IHC_19011506_876.jpg

GTX101820 IHC-P Image

Immunohistochemical analysis of paraffin-embedded D54 xenograft, using Ku70(GTX101820) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101820_41990_20150518_IHC-P_19011506_703.jpg

GTX101820 IHC-P Image

Ku70 antibody detects Ku70 protein at nucleus in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
Ku70 antibody (GTX101820) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101820_41892_20141121_WB_19011506_341.jpg

GTX101820 WB Image

Ku70 antibody detects Ku70 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku70 antibody (GTX101820) diluted by 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169