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Ku70 antibody

Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX64359
Anti-Ku70 antibody used in Immunoprecipitation (IP). GTX64359
Anti-Ku70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64359
Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX64359
Anti-Ku70 antibody used in Western Blot (WB). GTX64359
Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX64359

Cat No. GTX64359

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 408-609 of human XRCC6 (NP_001460.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-ray repair cross complementing 6 , CTC75 , CTCBF , G22P1 , KU70 , ML8 , TLAA

Cellular Localization

Nucleus

Background

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX64359

GTX64359 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX64359 Ku70 antibody.
Dilution : 1:100

Anti-Ku70 antibody used in Immunoprecipitation (IP). GTX64359

GTX64359 IP Image

IP analysis of SW480 cell lysate using GTX64359 Ku70 antibody.
Antibody amount : 3μg / 100μg lysate
Dilution : 1:1000

Anti-Ku70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64359

GTX64359 ICC/IF Image

ICC/IF analysis of A549 cells using GTX64359 Ku70 antibody.
Blue : DAPI

Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX64359

GTX64359 IHC-P Image

IHC-P analysis of human uterus tissue using GTX64359 Ku70 antibody.
Dilution : 1:100

Anti-Ku70 antibody used in Western Blot (WB). GTX64359

GTX64359 WB Image

WB analysis of various sample lysates using GTX64359 Ku70 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Ku70 antibody used in IHC (Paraffin sections) (IHC-P). GTX64359

GTX64359 IHC-P Image

IHC-P analysis of rat brain tissue using GTX64359 Ku70 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339