GeneTex
United States (US)

Ku70 antibody

GTX64359_20180530_IP_18121411_400.jpg
GTX64359_20180530_WB_18121411_366.jpg
GTX64359_20180530_ICCIF_18121411_237.jpg

Cat No. GTX64359

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Mouse, Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

70 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant funsion protein containing a sequence corresponding to amino acids 408-609 of human XRCC6 (NP_001460.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CTCBF Antibody , KU70 Antibody , CTC75 Antibody , ML8 Antibody , XRCC6 Antibody , TLAA Antibody , G22P1 Antibody

Background

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX64359_20180530_IP_18121411_400.jpg

GTX64359 IP Image

IP analysis of SW480 cell lysate using GTX64359 Ku70 antibody.
IP reaction : 3μg antibody / 100μg cell lysate
Dilution : 1:1000

GTX64359_20180530_WB_18121411_366.jpg

GTX64359 WB Image

WB analysis of various samples using GTX64359 Ku70 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX64359_20180530_ICCIF_18121411_237.jpg

GTX64359 ICC/IF Image

ICC/IF analysis of A549 cell using GTX64359 Ku70 antibody.
Blue : DAPI

Package List Price ($)
$ 319