APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500 - 1:2000 |
1:50 - 1:100 |
1:50 - 1:100 |
1:20 - 1:50 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 408-609 of human XRCC6 (NP_001460.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
X-ray repair cross complementing 6 , CTC75 , CTCBF , G22P1 , KU70 , ML8 , TLAA
Cellular Localization
Nucleus
Background
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX64359 IHC-P Image
IHC-P analysis of human gastric cancer tissue using GTX64359 Ku70 antibody. Dilution : 1:100
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GTX64359 IP Image
IP analysis of SW480 cell lysate using GTX64359 Ku70 antibody. Antibody amount : 3μg / 100μg lysate Dilution : 1:1000
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GTX64359 ICC/IF Image
ICC/IF analysis of A549 cells using GTX64359 Ku70 antibody. Blue : DAPI
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GTX64359 IHC-P Image
IHC-P analysis of human uterus tissue using GTX64359 Ku70 antibody. Dilution : 1:100
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GTX64359 WB Image
WB analysis of various sample lysates using GTX64359 Ku70 antibody. Dilution : 1:1000 Loading : 25μg per lane
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GTX64359 IHC-P Image
IHC-P analysis of rat brain tissue using GTX64359 Ku70 antibody. Dilution : 1:100
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REFERENCE
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REVIEW
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