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Ku70 antibody

Orthogonal
Anti-Ku70 antibody used in Western Blot (WB). GTX70270

Cat No. GTX70270

Reference 2
1

Host

 Mouse

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($329)
See all Ku70 products

APPLICATION

Application Note

For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

70 kDa. ( Note )

Positive Control

HeLa, Raji

PROPERTIES

Form

Liquid

Buffer

Serum diluted with PBS pH7.4

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant antigen containing the amino terminal portion of human Ku70 protein

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_372047

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-ray repair cross complementing 6 , CTC75 , CTCBF , G22P1 , KU70 , ML8 , TLAA

Cellular Localization

Nucleus

Background

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ku70 antibody used in Western Blot (WB). GTX70270

GTX70270 WB Image

Detection of human Ku70 protein by Western Blot using GeneTex Ku70 polyclonal antiserum (GTX70270) in HeLa whole cell extract (lane 1) and Raji whole cell lysate (lane 2).

REFERENCE

Application Reference

Keka IS et al. Nucleic Acids Res 2015; 43 (13) : 6359-72 Smarcal1 promotes double-strand-break repair by nonhomologous end-joining.
Application : WB
Reactivity : Human
Richard O Adeyemi et al. PLoS Pathog 2010; 6 (10) : e1001141 Parvovirus minute virus of mice induces a DNA damage response that facilitates viral replication.
Application : WB
Reactivity : Human
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REVIEW

Ku70 antibody Cat No. GTX70270

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Ku70 antibody used in Western Blot (WB). GTX70270
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS Pathog in 2010. PMID: 20949077
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SDS
PBS.pdf
Package List Price ($)
$ 329