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Ku80 antibody [149.8]

Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276
Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276
Anti-Ku80 antibody [149.8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70276
Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276

Cat No. GTX70276

Host

Mouse

Clonality

Monoclonal

Clone Name

149.8

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant antigen containing the carboxy terminal portion of human Ku80 protein

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-Ray Repair Cross Complementing 5 , Karp-1 , Karp1 , Ku80 , Kub2 , Ku86 , Nfiv , Xrcc5

Background

Originally identified as an autoantigen, the Ku protein are nuclear proteins found in eukaryotes. They exist as a heterodimer consisiting of Ku 70 and Ku 80, migrating at approximately 70 and 80 kDa respectively. They bind to DNA ends at double strand breaks. The Ku proteins interact with the DNA-dependent protein kinase (DNA-PK) and participate in the targeting of DNA-PK to DNA. The Ku proteins may participate in numerous cellular processes, including DNA repair and replication, cell signalling, cell proliferation, transcriptional activation and apoptosis.

Database

Research Area

DATA IMAGES

Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276

GTX70276 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
7.5% SDS PAGE
GTX70276 diluted at 1:10000

Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276

GTX70276 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
7.5% SDS PAGE
GTX70276 diluted at 1:10000

Anti-Ku80 antibody [149.8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70276

GTX70276 ICC/IF Image

Ku80 antibody [149.8] detects Ku80 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ku80 protein stained by Ku80 antibody [149.8] (GTX70276) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-Ku80 antibody [149.8] used in Western Blot (WB). GTX70276

GTX70276 WB Image

Detection of human Ku80 protein in HeLa whole cell lysate using GeneTex Ku80 149.8 monoclonal antibody GTX70276.

REFERENCE

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329