Ku80 antibody [5C5]

Cat. No. GTX60468

Host	Mouse
Clonality	Monoclonal
Clone Name	5C5
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Mouse
Package	100 μl ($409)

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Datasheet
Document

View product citations for antibody GTX60468 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human XRCC5 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

X-ray repair cross complementing 5 , KARP-1 , KARP1 , KU80 , KUB2 , Ku86 , NFIV

Cellular Localization

Nucleus,Nucleolus

Background

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]

Database

Gene ID: 7520 XRCC5
UniProt: P13010 XRCC5

Research Area

DATA IMAGES

GTX60468 FACS Image

FACS analysis of HeLa cells using GTX60468 Ku80 antibody [5C5].
Green : Ku80
Purple : negative control

GTX60468 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60468 Ku80 antibody [5C5].
Green : Ku80
Red: Actin filaments

GTX60468 IHC-P Image

IHC-P analysis of human tonsil tissue (left) and human colon cancer tissue (right) using GTX60468 Ku80 antibody [5C5].

GTX60468 ELISA Image

ELISA analysis of antigen using GTX60468 Ku80 antibody [5C5].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

GTX60468 WB Image

WB analysis of HeLa (1), MCF-7 (2), A549 (3) and NIH3T3 (4) cell lysate using GTX60468 Ku80 antibody [5C5].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 409

Qty