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Ku80 antibody [8H1]

Anti-Ku80 antibody [8H1] used in Western Blot (WB). GTX66843
Anti-Ku80 antibody [8H1] used in Immunoprecipitation (IP). GTX66843
Anti-Ku80 antibody [8H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66843

Cat No. GTX66843

Host

Mouse

Clonality

Monoclonal

Clone Name

8H1

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Monkey
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:100-500
IP 1:50-1:200
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine pH 7.4, 150 mM NaCl, 0.2% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human XRCC5 protein fragments expressed in E.coli.

Purification

Purified by antigen-affinity chromatography

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-ray repair cross complementing 5 , KARP-1 , KARP1 , KU80 , KUB2 , Ku86 , NFIV

Cellular Localization

Nucleus,Nucleus, nucleolus

Background

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ku80 antibody [8H1] used in Western Blot (WB). GTX66843

GTX66843 WB Image

WB analysis of COS7, Jurkat, HeLa, MCF7, and A549 cell lysates using GTX66843 Ku80 antibody [8H1].
Dilution : 1:1000

Anti-Ku80 antibody [8H1] used in Immunoprecipitation (IP). GTX66843

GTX66843 IP Image

IP analysis of HeLa cell lysate using GTX66843 Ku80 antibody [8H1].

Anti-Ku80 antibody [8H1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66843

GTX66843 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX66843 Ku80 antibody [8H1].
Dilution : 1:400

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399