Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku80 antibody [9403] (GTX70274) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
D: 293T 1ug
7.5% SDS PAGE
GTX70274 diluted at 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
7.5% SDS PAGE
GTX70274 diluted at 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX70274 at a 1/2000 dilution staining ~ 80 kDa Ku80 in 35mg of T24 whole cell extract. ECL detection.

Ku80 antibody [9403] detects Ku80 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Ku80 stained by Ku80 antibody [9403] (GTX70274) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (GTX102079) diluted at 1:1000.