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Ku80 antibody [9403]

Anti-Ku80 antibody [9403] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70274
Anti-Ku80 antibody [9403] used in Western Blot (WB). GTX70274
Anti-Ku80 antibody [9403] used in Western Blot (WB). GTX70274
Anti-Ku80 antibody [9403] used in Western Blot (WB). GTX70274

Cat No. GTX70274

Host

Mouse

Clonality

Monoclonal

Clone Name

9403

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:5000
ICC/IF 1:1000
Not tested in other applications.

Calculated MW

80 kDa. ( Note )

Positive Control

T24 , Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant antigen containing the amino terminal portion of human Ku80 protein

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-Ray Repair Cross Complementing 5 , Karp-1 , Karp1 , Ku80 , Kub2 , Ku86 , Nfiv , Xrcc5

Cellular Localization

Nuclear

Background

Originally identified as an autoantigen, the Ku protein are nuclear proteins found in eukaryotes. They exist as a heterodimer consisiting of Ku 70 and Ku 80, migrating at approximately 70 and 80 kDa respectively. They bind to DNA ends at double strand breaks. The Ku proteins interact with the DNA-dependent protein kinase (DNA-PK) and participate in the targeting of DNA-PK to DNA. The Ku proteins may participate in numerous cellular processes, including DNA repair and replication, cell signalling, cell proliferation, transcriptional activation and apoptosis.

Database

Research Area

DATA IMAGES

Anti-Ku80 antibody [9403] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70274

GTX70274 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Ku80(GTX70274) antibody (green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2000.

Anti-Ku80 antibody [9403] used in Western Blot (WB). GTX70274

GTX70274 WB Image

GTX70274 at a 1/2000 dilution staining ~ 80 kDa Ku80 in 35mg of T24 whole cell extract. ECL detection.

Anti-Ku80 antibody [9403] used in Western Blot (WB). GTX70274

GTX70274 WB Image

Sample (30 μg of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
7.5% SDS PAGE
GTX70274 diluted at 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Ku80 antibody [9403] used in Western Blot (WB). GTX70274

GTX70274 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
D: 293T 1ug
7.5% SDS PAGE
GTX70274 diluted at 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329