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Ku80 antibody [9403]

Anti-Ku80 antibody [9403] used in Western Blot (WB). GTX70274
Anti-Ku80 antibody [9403] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70274
Anti-Ku80 antibody [9403] used in Western Blot (WB). GTX70274
Anti-Ku80 antibody [9403] used in Western Blot (WB). GTX70274
Anti-Ku80 antibody [9403] used in Western Blot (WB). GTX70274

Cat. No. GTX70274

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 9403

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all Ku80 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:5000
ICC/IF 1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

T24 , Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant antigen containing the amino terminal portion of human Ku80 protein

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_372051

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

X-ray repair cross complementing 5 , KARP-1 , KARP1 , KU80 , KUB2 , Ku86 , NFIV

Cellular Localization

Nucleus , Nucleolus

Background

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ku80 antibody [9403] used in Western Blot (WB). GTX70274

GTX70274 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ku80 antibody [9403] (GTX70274) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Ku80 antibody [9403] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70274

GTX70274 ICC/IF Image

Ku80 antibody [9403] detects Ku80 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Ku80 stained by Ku80 antibody [9403] (GTX70274) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (GTX102079) diluted at 1:1000.

Anti-Ku80 antibody [9403] used in Western Blot (WB). GTX70274

GTX70274 WB Image

GTX70274 at a 1/2000 dilution staining ~ 80 kDa Ku80 in 35mg of T24 whole cell extract. ECL detection.
Anti-Ku80 antibody [9403] used in Western Blot (WB). GTX70274

GTX70274 WB Image

Sample (30 μg of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
7.5% SDS PAGE
GTX70274 diluted at 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Ku80 antibody [9403] used in Western Blot (WB). GTX70274

GTX70274 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
D: 293T 1ug
7.5% SDS PAGE
GTX70274 diluted at 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations