PRODUCT
Summary
This antibody was validated on 76 different Normal and cancerTissues by IHC-P.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Tonsil: A strong staining should be seen in the nuclei of all cells.
Negative Control
A tumor or a cell line with documented loss of Ku80 expression.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human XRCC5 fragmen
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
X-ray repair cross complementing 5 , KARP-1 , KARP1 , KU80 , KUB2 , Ku86 , NFIV
Cellular Localization
Nucleus , Nucleolus
Background
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480](/templates/default/js/lightbox/images/loading.gif "Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480")
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GTX04480 IHC-P Image
IHC-P analysis of human esophageal squamous cell carcinoma tissue section using GTX04480 Ku80 antibody [MSVA-880M] HistoMAX™.
Esophagus Squamous cell carcinoma with reduced Ku80 staining of tumor cells as compared to stroma cells.
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GTX04480 IHC-P Image
IHC-P analysis of human appendix mucosa tissue section using GTX04480 Ku80 antibody [MSVA-880M] HistoMAX™.
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GTX04480 IHC-P Image
IHC-P analysis of human skin tissue section using GTX04480 Ku80 antibody [MSVA-880M] HistoMAX™.
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REFERENCE
There are currently no references for Ku80 antibody [MSVA-880M] HistoMAX™ (GTX04480). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Ku80 antibody [MSVA-880M] HistoMAX™ (GTX04480). Be the first to share your experience with this product.
![Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480](/upload/website/prouct_img/small/GTX04480/GTX04480_20230728_IHC-P_389_23072723_468.webp "Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480")
![Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480](/upload/website/prouct_img/small/GTX04480/GTX04480_20230728_IHC-P_129_23072722_291.webp "Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480")
![Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480](/upload/website/prouct_img/small/GTX04480/GTX04480_20230728_IHC-P_259_23072722_323.webp "Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480")
![Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480](/upload/website/prouct_img/normal/GTX04480/GTX04480_20230728_IHC-P_389_23072723_468.webp "Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480")
![Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480](/upload/website/prouct_img/normal/GTX04480/GTX04480_20230728_IHC-P_129_23072722_291.webp "Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480")
![Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480](/upload/website/prouct_img/normal/GTX04480/GTX04480_20230728_IHC-P_259_23072722_323.webp "Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480")