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Ku80 antibody [MSVA-880M] HistoMAX™

HistoMAX
Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480
Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480
Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480

Cat. No. GTX04480

Host

Mouse

Clonality

Multiclonal

Clone Name

MSVA-880M

Isotype

IgG1, IgG2b

Application

IHC-P

Reactivity

Human
Package
500 μl ($679),
100 μl ($299)
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.

Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

Tonsil: A strong staining should be seen in the nuclei of all cells.

Negative Control

A tumor or a cell line with documented loss of Ku80 expression.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human XRCC5 fragmen

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

X-ray repair cross complementing 5 , KARP-1 , KARP1 , KU80 , KUB2 , Ku86 , NFIV

Cellular Localization

Nucleus , Nucleolus

Background

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480

GTX04480 IHC-P Image

IHC-P analysis of human mucosa from appendix tissue using GTX04480 Ku80 antibody [MSVA-880M] HistoMAX™.
Appendix mucosa showing a strong nuclear Ku80 staining in all cells.

Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480

GTX04480 IHC-P Image

IHC-P analysis of human skin tissue using GTX04480 Ku80 antibody [MSVA-880M] HistoMAX™.
Strong nuclear Ku80 staining in all cells of the skin.

Anti-Ku80 antibody [MSVA-880M] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04480

GTX04480 IHC-P Image

IHC-P analysis of human esophageal squamous cell carcinoma (ESCC) tissue using GTX04480 Ku80 antibody [MSVA-880M] HistoMAX™.
Esophagus Squamous cell carcinoma with reduced Ku80 staining of tumor cells as compared to stroma cells.

REFERENCE

There are currently no references for Ku80 antibody [MSVA-880M] HistoMAX™ (GTX04480). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 679
$ 299