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Ku80 antibody

Anti-Ku80 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33290
Anti-Ku80 antibody used in Western Blot (WB). GTX33290
Anti-Ku80 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33290
Anti-Ku80 antibody used in IHC (Paraffin sections) (IHC-P). GTX33290
Anti-Ku80 antibody used in Immunoprecipitation (IP). GTX33290
Anti-Ku80 antibody used in IHC (Paraffin sections) (IHC-P). GTX33290

Cat No. GTX33290

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:20-1:100
IHC-P 1:100-1:200
IP 1:50-1:200
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 543-732 of human XRCC5 (NP_066964.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

X-ray repair cross complementing 5 , KARP-1 , KARP1 , KU80 , KUB2 , Ku86 , NFIV

Cellular Localization

Nucleus,Nucleolus

Background

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ku80 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33290

GTX33290 ICC/IF Image

ICC/IF analysis of A549 cells using GTX33290 Ku80 antibody.
Blue : DAPI

Anti-Ku80 antibody used in Western Blot (WB). GTX33290

GTX33290 WB Image

WB analysis of various sample lysates using GTX33290 Ku80 antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-Ku80 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33290

GTX33290 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33290 Ku80 antibody.
Blue : DAPI
Dilution : 1:100

Anti-Ku80 antibody used in IHC (Paraffin sections) (IHC-P). GTX33290

GTX33290 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX33290 Ku80 antibody.
Dilution : 1:100

Anti-Ku80 antibody used in Immunoprecipitation (IP). GTX33290

GTX33290 IP Image

IP analysis of 293T cells using GTX33290 Ku80 antibody.
IP reaction : 200μg extracts / 1μg antibody antibody
Dilution : 1:1000

Anti-Ku80 antibody used in IHC (Paraffin sections) (IHC-P). GTX33290

GTX33290 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX33290 Ku80 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329