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L-FABP antibody [N1C3]

Anti-L-FABP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109142
Anti-L-FABP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109142
Anti-L-FABP antibody [N1C3] used in IHC (Frozen sections) (IHC-Fr). GTX109142
Anti-L-FABP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109142
Anti-L-FABP antibody [N1C3] used in Western Blot (WB). GTX109142

Cat. No. GTX109142

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human L-FABP Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950246

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

fatty acid binding protein 1 , FABPL , L-FABP

Cellular Localization

Cytoplasm

Background

FABP1 encodes the fatty acid binding protein found in liver. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABP1 and FABP6 (the ileal fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-L-FABP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109142

GTX109142 IHC-P Image

L-FABP antibody [N1C3] detects L-FABP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
L-FABP stained by L-FABP antibody [N1C3] (GTX109142) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-L-FABP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109142

GTX109142 IHC-P Image

L-FABP antibody [N1C3] detects L-FABP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
L-FABP stained by L-FABP antibody [N1C3] (GTX109142) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-L-FABP antibody [N1C3] used in IHC (Frozen sections) (IHC-Fr). GTX109142

GTX109142 IHC-Fr Image

L-FABP antibody [N1C3] detects L-FABP protein at cytoplasm in rat kidney by immunohistochemical analysis.
Sample: Frozen section of rat kidney.
L-FABP antibody [N1C3] (GTX109142) diluted at 1:200.

Anti-L-FABP antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109142

GTX109142 IHC-P Image

liver FABP antibody [N1C3] detects liver FABP protein at cytoplasm on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
liver FABP antibody [N1C3] (GTX109142) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-L-FABP antibody [N1C3] used in Western Blot (WB). GTX109142

GTX109142 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with L-FABP antibody [N1C3] (GTX109142) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

L-FABP antibody [N1C3] Cat. No. GTX109142

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 12-Apr-2018
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : liver (hepatocellular adenoma)
Blocking : H2O2 Endogenous peroxidase, 37°C, 0.07Hr
Permeabilization /Antigen retrieval : 100°C, 64 minHr
Primary Antibody : 1:500, 37°C, 0.5Hr
Figure Note : The staining was performed with the automatic Ventana Systems (BenchMark ULTRA).
Date : Anonymous submitted on 19-Apr-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 30μg
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169