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L-Plastin antibody

Anti-L-Plastin antibody used in Western Blot (WB). GTX105789
Anti-L-Plastin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105789
Anti-L-Plastin antibody used in Western Blot (WB). GTX105789
Anti-L-Plastin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105789
Anti-L-Plastin antibody used in IHC (Paraffin sections) (IHC-P). GTX105789
Anti-L-Plastin antibody used in Western Blot (WB). GTX105789

Cat. No. GTX105789

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all L-Plastin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.28 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human L-Plastin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721688

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lymphocyte cytosolic protein 1 , CP64 , HEL-S-37 , L-PLASTIN , LC64P , LPL , PLS2

Cellular Localization

Cytoplasm

Background

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-L-Plastin antibody used in Western Blot (WB). GTX105789

GTX105789 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody (GTX105789) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-L-Plastin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105789

GTX105789 ICC/IF Image

L-Plastin antibody detects L-Plastin protein at cytoplasm and focal adhesion site by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: L-Plastin protein stained by L-Plastin antibody (GTX105789) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-L-Plastin antibody used in Western Blot (WB). GTX105789

GTX105789 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody (GTX105789) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-L-Plastin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105789

GTX105789 ICC/IF Image

L-Plastin antibody detects L-Plastin protein by immunofluorescent analysis.
Sample: A549 cells were fixed in ice-cold MeOH for 5 min.
Green: L-Plastin stained by L-Plastin antibody (GTX105789) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-L-Plastin antibody used in IHC (Paraffin sections) (IHC-P). GTX105789

GTX105789 IHC-P Image

L-Plastin antibody detects L-Plastin protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
L-Plastin stained by L-Plastin antibody (GTX105789) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-L-Plastin antibody used in Western Blot (WB). GTX105789

GTX105789 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with L-Plastin antibody (GTX105789) diluted at 1:10000.

REFERENCE

Application Reference

Vanhunsel S et al. NPJ Aging Mech Dis 2021; 7 (1) : 22 The killifish visual system as an in vivo model to study brain aging and rejuvenation.
Application : IHC-Fr
Timur Alexander Yorgan et al. Bone 2019; 130 : 115062 Mice lacking plastin-3 display a specific defect of cortical bone acquisition.
Application : WB
Chen JY et al. J Nutr Biochem 2013; 24 (11) : 1889-910 High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169