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United States (US)

L-Plastin antibody

Anti-L-Plastin antibody used in Immunoprecipitation (IP). GTX33300
Anti-L-Plastin antibody used in Western Blot (WB). GTX33300

Cat No. GTX33300

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

70 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human LCP1 (NP_002289.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lymphocyte Cytosolic Protein 1,Cp64,Hel-S-37,L-Plastin,Lc64P,Lpl,Pls2,Lcp1

Background

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-L-Plastin antibody used in Immunoprecipitation (IP). GTX33300

GTX33300 IP Image

IP analysis of Jurkat cell lysate using GTX33300 L-Plastin antibody.
IP reaction : 3μg antibody / 150μg cell lysate
Dilution : 1:1000

Anti-L-Plastin antibody used in Western Blot (WB). GTX33300

GTX33300 WB Image

WB analysis of various samples using GTX33300 L-Plastin antibody.
Dilution : 1:1000
Loading : 25μg per lane

Package List Price ($)
$ 329