APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:2000 |
1:20 - 1:50 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human LCP1 (NP_002289.2).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
lymphocyte cytosolic protein 1 , CP64 , HEL-S-37 , L-PLASTIN , LC64P , LPL , PLS2
Cellular Localization
Cytoplasm, cytoskeleton,Cell junction
Background
Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX33300 IP Image
IP analysis of Jurkat cell lysate using GTX33300 L-Plastin antibody. Antibody amount : 3μg / 150μg lysate Dilution : 1:1000
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GTX33300 WB Image
WB analysis of various sample lysates using GTX33300 L-Plastin antibody. Dilution : 1:1000 Loading : 25μg per lane
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REFERENCE
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REVIEW
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