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L1CAM (150kDa subunit) antibody

Anti-L1CAM (150kDa subunit) antibody used in Western Blot (WB). GTX129010
Anti-L1CAM (150kDa subunit) antibody used in IHC (Paraffin sections) (IHC-P). GTX129010

Cat No. GTX129010

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

140 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human L1CAM (150kDa subunit). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

L1 cell adhesion molecule , CAML1 , CD171 , HSAS , HSAS1 , MASA , MIC5 , N-CAM-L1 , N-CAML1 , NCAM-L1 , S10 , SPG1

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES

Anti-L1CAM (150kDa subunit) antibody used in Western Blot (WB). GTX129010

GTX129010 WB Image

L1CAM (150kDa subunit) antibody detects L1CAM (150kDa subunit) protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract |
C. 30 μg IMR32 whole cell lysate/extract
D. 30 μg SK-N-AS whole cell lysate/extract
5 % SDS-PAGE
L1CAM (150kDa subunit) antibody (GTX129010) dilution: 1:1000

Anti-L1CAM (150kDa subunit) antibody used in IHC (Paraffin sections) (IHC-P). GTX129010

GTX129010 IHC-P Image

L1CAM (150kDa subunit) antibody detects L1CAM (150kDa subunit) protein at cell membrane on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
L1CAM (150kDa subunit) antibody (GTX129010) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169