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L1CAM antibody [1A8]

KO/KD Overexpression
Anti-L1CAM antibody [1A8] used in Flow cytometry (FACS). GTX84236
Anti-L1CAM antibody [1A8] used in Flow cytometry (FACS). GTX84236
Anti-L1CAM antibody [1A8] used in Western Blot (WB). GTX84236
Anti-L1CAM antibody [1A8] used in Western Blot (WB). GTX84236
Anti-L1CAM antibody [1A8] used in Western Blot (WB). GTX84236
Anti-L1CAM antibody [1A8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84236

Cat. No. GTX84236

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1A8

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Monkey
Package
100 μl ($519)
See all L1CAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

140 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human L1CAM (NP_000416) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

L1 cell adhesion molecule , CAML1 , CD171 , HSAS , HSAS1 , MASA , MIC5 , N-CAM-L1 , N-CAML1 , NCAM-L1 , S10 , SPG1

Cellular Localization

Cell membrane,Cell projection, dendrite

Background

Cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons.

Database

Research Area

DATA IMAGES

Anti-L1CAM antibody [1A8] used in Flow cytometry (FACS). GTX84236

GTX84236 FACS Image

FACS analysis of Jurkat cells using GTX84236 L1CAM antibody [1A8].
Red : Primary antibody
Blue : Negative control antibody

Anti-L1CAM antibody [1A8] used in Flow cytometry (FACS). GTX84236

GTX84236 FACS Image

FACS analysis of HEK293T cells transfected with either L1CAM plasmid(Red) or empty vector control plasmid(Blue) using GTX84236 L1CAM antibody [1A8].

Anti-L1CAM antibody [1A8] used in Western Blot (WB). GTX84236

GTX84236 WB Image

Overexpression

WB analysis of HEK293T cells transfected with L1CAM plasmid (Right) or empty vector (Left) for 48 hrs using GTX84236 L1CAM antibody [1A8].
Loading : 5 ug per lane

Anti-L1CAM antibody [1A8] used in Western Blot (WB). GTX84236

GTX84236 WB Image

WB analysis of various cell lines using GTX84236 L1CAM antibody [1A8].
Loading : 35 ug per lane
Dilution : 1:200

Anti-L1CAM antibody [1A8] used in Western Blot (WB). GTX84236

GTX84236 WB Image

KO/KD

WB analysis of wild type and L1CAM knock out HeLa cell lysate(10μg per lane) using GTX84236 L1CAM antibody [1A8].
Dilution : 1:500

Anti-L1CAM antibody [1A8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84236

GTX84236 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with L1CAM plasmid using GTX84236 L1CAM antibody [1A8].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519