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L1CAM antibody [2C7]

Overexpression
Anti-L1CAM antibody [2C7] used in Flow cytometry (FACS). GTX84241
Anti-L1CAM antibody [2C7] used in IHC (Paraffin sections) (IHC-P). GTX84241
Anti-L1CAM antibody [2C7] used in Western Blot (WB). GTX84241
Anti-L1CAM antibody [2C7] used in Western Blot (WB). GTX84241
Anti-L1CAM antibody [2C7] used in Immunoprecipitation (IP). GTX84241
Anti-L1CAM antibody [2C7] used in Flow cytometry (FACS). GTX84241
Anti-L1CAM antibody [2C7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84241
Anti-L1CAM antibody [2C7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84241

Cat. No. GTX84241

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2C7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP

Reactivity

 Human, Monkey
Package
100 μl ($519)
See all L1CAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:50-1:100
IHC-P 1:50
FACS 1:100
IP 2-4ug/mg
Not tested in other applications.

Calculated MW

140 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human L1CAM (NP_000416) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

L1 cell adhesion molecule , CAML1 , CD171 , HSAS , HSAS1 , MASA , MIC5 , N-CAM-L1 , N-CAML1 , NCAM-L1 , S10 , SPG1

Cellular Localization

Cell membrane,Cell projection, dendrite

Background

Cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons.

Database

Research Area

DATA IMAGES

Anti-L1CAM antibody [2C7] used in Flow cytometry (FACS). GTX84241

GTX84241 FACS Image

FACS analysis of HeLa cells using GTX84241 L1CAM antibody [2C7].
Red : Primary antibody
Blue : Negative control antibody

Anti-L1CAM antibody [2C7] used in IHC (Paraffin sections) (IHC-P). GTX84241

GTX84241 IHC-P Image

IHC-P analysis of human liver tissue using GTX84241 L1CAM antibody [2C7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-L1CAM antibody [2C7] used in Western Blot (WB). GTX84241

GTX84241 WB Image

WB analysis of various cell lines using GTX84241 L1CAM antibody [2C7].
Loading : 35 ug per lane
Dilution : 1:200

Anti-L1CAM antibody [2C7] used in Western Blot (WB). GTX84241

GTX84241 WB Image

Overexpression

WB analysis of HEK293T cells transfected with L1CAM plasmid (Right) or empty vector (Left) for 48 hrs using GTX84241 L1CAM antibody [2C7].
Loading : 5 ug per lane

Anti-L1CAM antibody [2C7] used in Immunoprecipitation (IP). GTX84241

GTX84241 IP Image

IP analysis of DDDDK tagged L1CAM overexpressed HEK293T lysate using GTX84241 L1CAM antibody [2C7].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody.
IP reaction : 2μg antibody / 500μl cell lysate

Anti-L1CAM antibody [2C7] used in Flow cytometry (FACS). GTX84241

GTX84241 FACS Image

FACS analysis of HEK293T cells transfected with either L1CAM plasmid(Red) or empty vector control plasmid(Blue) using GTX84241 L1CAM antibody [2C7].

Anti-L1CAM antibody [2C7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84241

GTX84241 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with L1CAM plasmid using GTX84241 L1CAM antibody [2C7].

Anti-L1CAM antibody [2C7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84241

GTX84241 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX84241 L1CAM antibody [2C7].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519