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L1CAM antibody [UJ127]

Anti-L1CAM antibody [UJ127] used in Immunohistochemistry (IHC). GTX23200

Cat. No. GTX23200

Reference 6

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 UJ127

Isotype

 IgG1

Application

 WB, IHC-P, IP, IHC

Reactivity

 Human, Rat
Package
500 μl ($399)
See all L1CAM products

APPLICATION

Application Note

Immunoprecipitation (natured and denatured): use at a concentration of 2μg / mg. Western Blotting: use at a concentration of 1 - 2μg / ml for 2hrs at RT. Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 1 - 2μg / ml for 30 min at RT. Optimal dilutions/concentrations should be determined by the end user. By Western blot, this antibody detects a band of 220-240 kDa, which corresponds to the predicted molecular weight of L1CAM. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Calculated MW

140 kDa. ( Note )

Positive Control

IMR-5 cells

Product Note

GTX23200 is useful in the identification of primitive neuroectodermal tumors. It binds to tumors of neuroectodermal and glial origin. It does not bind to pediatric or adult brain.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Homogenous suspension of 16 week human fetal brain.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_384977

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

L1 cell adhesion molecule , CAML1 , CD171 , HSAS , HSAS1 , MASA , MIC5 , N-CAM-L1 , N-CAML1 , NCAM-L1 , S10 , SPG1

Cellular Localization

Cell membrane , Cell projection, dendrite

Background

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES

Anti-L1CAM antibody [UJ127] used in Immunohistochemistry (IHC). GTX23200

GTX23200 IHC Image

GTX23200 at a dilution of 1/1000, staining L1CAM (green; Alexa 488 secondary at 1/2000) on 30m coronal rat brain tissue sections in free floating IHC. Images showing neuron body, cytoplasm and axon labeling: [A] neuron; 40x objective [B] neuron and axons;

REFERENCE

Application Reference

Cheng L et al. EMBO J 2011; 30 (5) : 800-13 L1CAM regulates DNA damage checkpoint response of glioblastoma stem cells through NBS1.
Application : WB
Reactivity : Human
Cheng L et al. Biochem Biophys Res Commun 2011; 406 (4) : 643-8 Elevated invasive potential of glioblastoma stem cells.
Application : WB
Li Y et al. Cancer Cell Int 2010; 10 : 34 Soluble L1CAM promotes breast cancer cell adhesion and migration in vitro, but not invasion.
Reactivity : Human

Clone Reference

Yang M et al. J Neurooncol 2011; 105 (1):27-44 L1 stimulation of human glioma cell motility correlates with FAK activation.
Yang M et al. Cancer Cell Int 2009; 9:27 Stimulation of glioma cell motility by expression, proteolysis, and release of the L1 neural cell recognition molecule.
Application : WB
Reactivity : Rat
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399