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L1CAM antibody

Anti-L1CAM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87010
Anti-L1CAM antibody used in Western Blot (WB). GTX87010

Cat. No. GTX87010

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μg ($399)
See all L1CAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
Not tested in other applications.

Calculated MW

140 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human L1CAM (1147-1196).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CAML1 , CD171 , HSAS , HSAS1 , L1 cell adhesion molecule , L1CAM , MASA , MIC5 , NCAML1 , S10 , SPG1

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]

Database

Research Area

DATA IMAGES

Anti-L1CAM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87010

GTX87010 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX87010 L1CAM antibody. The picture on the right is blocked with the synthesized peptide.

Anti-L1CAM antibody used in Western Blot (WB). GTX87010

GTX87010 WB Image

WB analysis of K562 cell lysates using GTX87010 L1CAM antibody. The lane on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399