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L3MBTL1 antibody

Anti-L3MBTL1 antibody used in Western Blot (WB). GTX103793
Anti-L3MBTL1 antibody used in Western Blot (WB). GTX103793
Anti-L3MBTL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103793

Cat No. GTX103793

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr

Reactivity

Human, Caenorhabditis elegans
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

HeLaS3 , human L3MBTL-transfected 293T

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human L3MBTL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

L3MBTL histone methyl-lysine binding protein 1 , H-L(3)MBT , L3MBTL , ZC2HC3 , dJ138B7.3

Cellular Localization

Nucleus

Background

This gene encodes the homolog of a protein identified in Drosophila as a suppressor of malignant transformation of neuroblasts and ganglion-mother cells in the optic centers of the brain. This gene product is localized to condensed chromosomes in mitotic cells. Overexpression of this gene in a glioma cell line results in improper nuclear segregation and cytokinesis producing multinucleated cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-L3MBTL1 antibody used in Western Blot (WB). GTX103793

GTX103793 WB Image

L3MBTL antibody detects L3MBTL protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human L3MBTL-transfected 293T cells
7.5 % SDS-PAGE
L3MBTL antibody (GTX103793) dilution: 1:5000

Anti-L3MBTL1 antibody used in Western Blot (WB). GTX103793

GTX103793 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX103793 diluted at 1:1000

Anti-L3MBTL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103793

GTX103793 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using L3MBTL(GTX103793) antibody at 1:200 dilution.

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169