GeneTex
United States (US)

L3MBTL1 antibody

Overexpression
GTX115782_40611_IFA_18121410_967.jpg
GTX115782_40611_WB_B_18121410_420.jpg
GTX115782_40611_WB_18121410_163.jpg

Cat No. GTX115782

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

84 kDa. ( Note )

Positive Control

HeLa nucleus , human L3MBTL-transfected 293T

Predict Reactivity

Mouse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human L3MBTL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

L3Mbtl1, Histone Methyl-Lysine Binding Protein,H-L(3)Mbt,L3Mbtl,Zc2Hc3,Dj138B7.3,L3Mbtl1

Cellular Localization

Nucleus

Background

This gene encodes the homolog of a protein identified in Drosophila as a suppressor of malignant transformation of neuroblasts and ganglion-mother cells in the optic centers of the brain. This gene product is localized to condensed chromosomes in mitotic cells. Overexpression of this gene in a glioma cell line results in improper nuclear segregation and cytokinesis producing multinucleated cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Database

DATA IMAGES
GTX115782_40611_IFA_18121410_967.jpg

GTX115782 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using L3MBTL(GTX115782) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX115782_40611_WB_B_18121410_420.jpg

GTX115782 WB Image

Overexpression

L3MBTL antibody detects L3MBTL protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg whole cell lysate/extract of human L3MBTL-transfected 293T cells
7.5 % SDS-PAGE
L3MBTL antibody (GTX115782) dilution: 1:5000

GTX115782_40611_WB_18121410_163.jpg

GTX115782 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa nucleus
7.5% SDS PAGE
GTX115782 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169