GeneTex
United States (US)

L3MBTL2 antibody

GTX107234_40009_IP_19011506_177.jpg
GTX107234_40009_ChIP_19011506_208.jpg
GTX107234_40009_20161013_WB_19011506_151.jpg
GTX107234_40009_20161013_WB_M_testis_19011506_731.jpg

Cat No. GTX107234

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP, ChIP assay
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:500-1:1000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

Positive Control

NT2D1 , mouse testis

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human L3MBTL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

L3Mbtl2, Polycomb Repressive Complex 1 Subunit,H-L(3)Mbt-L,L3Mbt,L3Mbtl2

Cellular Localization

Nucleus

Background

Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Its association with a chromatin-remodeling complex suggests that it may contribute to prevent expression of genes that trigger the cell into mitosis. Binds to monomethylated and dimethylated 'Lys-20' on histone H4. Binds histone H3 peptides that are monomethylated or dimethylated on 'Lys-4', 'Lys-9' or 'Lys-27'.

Database

DATA IMAGES
GTX107234_40009_IP_19011506_177.jpg

GTX107234 IP Image

L3MBTL2 antibody immunoprecipitates L3MBTL2 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of L3MBTL2 protein by 2.5 μg of L3MBTL2 antibody (GTX107234) 5% SDS-PAGE The immunoprecipitated L3MBTL2 protein was detected by L3MBTL2 antibody (GTX107234) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX107234_40009_ChIP_19011506_208.jpg

GTX107234 ChIP assay Image

L3MBTL2 antibody immunoprecipitates L3MBTL2 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of L3MBTL2 antibody (GTX107234) The precipitated DNA was detected by PCR with primer set targeting to CDC7 gene locus.

GTX107234_40009_20161013_WB_19011506_151.jpg

GTX107234 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with L3MBTL2 antibody (GTX107234) diluted at 1:500.

GTX107234_40009_20161013_WB_M_testis_19011506_731.jpg

GTX107234 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with L3MBTL2 antibody (GTX107234) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169