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L3MBTL3 antibody

Anti-L3MBTL3 antibody used in Western Blot (WB). GTX32696
Anti-L3MBTL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32696
Anti-L3MBTL3 antibody used in Immunoprecipitation (IP). GTX32696

Cat No. GTX32696

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:100
IP 1:50-1:200
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human L3MBTL3 (NP_001007103.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

L3MBTL histone methyl-lysine binding protein 3 , MBT-1 , MBT1

Cellular Localization

Nucleus

Background

This gene encodes a member of the malignant brain tumor (MBT) family of chromatin interacting transcriptional repressors. Members of this family function as methyl-lysine readers, which recognize methylated lysine residues on histone protein tails, and are associated with the repression of gene expression. The encoded protein may regulate hematopoiesis. Homozygous deletion of this gene has been observed in human patients with medulloblastoma. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES

Anti-L3MBTL3 antibody used in Western Blot (WB). GTX32696

GTX32696 WB Image

WB analysis of various sample lysates using GTX32696 L3MBTL3 antibody. The signal was developed with ECL plus-Enhanced.
Loading : 25ug per lane
Dilution : 1:1000

Anti-L3MBTL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32696

GTX32696 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX32696 L3MBTL3 antibody.
Blue : DAPI

Anti-L3MBTL3 antibody used in Immunoprecipitation (IP). GTX32696

GTX32696 IP Image

IP analysis of HeLa cells using GTX32696 L3MBTL3 antibody.
IP reaction : 200μg extracts / 1μg antibody antibody
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329