GeneTex

  • Country / Location Selection

United States (US)

LAG3 antibody [C9B7W] (Low endotoxin, azide free)

Anti-LAG3 antibody [C9B7W] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX42244

Cat. No. GTX42244

Reference 2

Host

 Rat

Clonality

 Monoclonal

Clone Name

 C9B7W

Isotype

 IgG1

Application

 FACS, Neutralizing/Inhibition

Reactivity

 Mouse
Package
500 μg ($749)
See all LAG3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 1/50-1/200
Neutralizing/Inhibition Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Neutralizing/Inhibition
Rat anti Mouse CD223 antibody, clone C9B7W is reported to block the in vitro function of murine LAG-3 but does not block binding of LAG-3 to MHC class II (Workman et al. 2002).
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Product Note

This antibody recognizes an epitope within the D2 domain of LAG3.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Murine LAG3 Ig fusion protein.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lymphocyte-activation gene 3 , CD223 , LAG-3 , Ly66

Cellular Localization

Membrane; Single-pass type I membrane protein

Database

Research Area

DATA IMAGES

Anti-LAG3 antibody [C9B7W] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX42244

GTX42244 FACS Image

FACS analysis of CD223 transfected cells using GTX42244 LAG3 antibody [C9B7W] (Low endotoxin, azide free).

REFERENCE

Clone Reference

Scarlett UK et al. J Exp Med 2012; 209 (3):495-506 Ovarian cancer progression is controlled by phenotypic changes in dendritic cells.
Meng Q et al. Invest Ophthalmol Vis Sci 2006; 47 (10):4444-52 CD4+PD-1+ T cells acting as regulatory cells during the induction of anterior chamber-associated immune deviation.
Submit a Reference

REVIEW

There are currently no reviews for LAG3 antibody [C9B7W] (Low endotoxin, azide free) (GTX42244). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 749
Custom Conjugations