GeneTex
United States (US)

LAG3 antibody, Internal

GTX81605_2367_WB_18121411_778.jpg
GTX81605_2369_WB_18121411_996.jpg
GTX81605_2371_WB_18121411_738.jpg
GTX81605_387_FACS_18121411_585.jpg
GTX81605_2368_WB_18121411_103.jpg
GTX81605_2370_WB_18121411_754.jpg
GTX81605_1150_IHC-P_18121411_335.jpg

Cat No. GTX81605

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, FACS
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
IHC-P 1:10-1:50
FACS 1:25
Not tested in other applications.

Calculated MW

57 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human LAG3.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Lymphocyte Activating 3,Cd223,Lag3

Background

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq]

Database

DATA IMAGES
GTX81605_2367_WB_18121411_778.jpg

GTX81605 WB Image

WB analysis of various samples using GTX81605 LAG3 antibody, Internal.
Lane 1: human ovary lysate
Lane 2: mouse liver lysate
Loading : 20 µg per lane
Dilution : 1:1000

GTX81605_2369_WB_18121411_996.jpg

GTX81605 WB Image

WB analysis of various samples using GTX81605 LAG3 antibody, Internal.
Lane 1: K562 whole cell lysates
Lane 2: human liver lysates
Lane 3: human ovary lysates
Loading : 20 µg per lane
Dilution : 1:2000

GTX81605_2371_WB_18121411_738.jpg

GTX81605 WB Image

WB analysis of mouse heart lysate using GTX81605 LAG3 antibody, Internal.
Loading : 20 µg per lane
Dilution : 1:2000

GTX81605_387_FACS_18121411_585.jpg

GTX81605 FACS Image

FACS analysis of Jurkat cells using GTX81605 LAG3 antibody, Internal.
Red : LAG3
Blue : Isotype control
Fixation : 2% paraformaldehyde (PFA) (10 min)
Permeabilization : 90% methanol (10 min)
Dilution : 1:25

GTX81605_2368_WB_18121411_103.jpg

GTX81605 WB Image

WB analysis of various samples using GTX81605 LAG3 antibody, Internal.
Lane 1: 293T/17 whole cell lysate
Lane 2: mouse heart lysate
Loading : 20 µg per lane
Dilution : 1:2000

GTX81605_2370_WB_18121411_754.jpg

GTX81605 WB Image

WB analysis of 293T/17 whole cell lysate using GTX81605 LAG3 antibody, Internal.
Loading : 20 µg per lane
Dilution : 1:2000

GTX81605_1150_IHC-P_18121411_335.jpg

GTX81605 IHC-P Image

IHC-P analysis of human thymus tissue using GTX81605 LAG3 antibody, Internal.
Antigen retrieval : heat-induced antigen retrieval with citrate buffer (pH6)
Dilution : 1:25

Package List Price ($)
$ 339