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LAG3 antibody, Internal

Anti-LAG3 antibody, Internal used in Western Blot (WB). GTX81605
Anti-LAG3 antibody, Internal used in Western Blot (WB). GTX81605
Anti-LAG3 antibody, Internal used in Western Blot (WB). GTX81605
Anti-LAG3 antibody, Internal used in Flow cytometry (FACS). GTX81605
Anti-LAG3 antibody, Internal used in Western Blot (WB). GTX81605
Anti-LAG3 antibody, Internal used in Western Blot (WB). GTX81605
Anti-LAG3 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81605

Cat. No. GTX81605

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human, Mouse
Package
400 μl ($399)
See all LAG3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:10-1:50
FACS 1:25
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human LAG3.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lymphocyte activating 3 , CD223

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-LAG3 antibody, Internal used in Western Blot (WB). GTX81605

GTX81605 WB Image

WB analysis of various samples using GTX81605 LAG3 antibody, Internal.
Lane 1: K562 whole cell lysates
Lane 2: human liver lysates
Lane 3: human ovary lysates
Loading : 20 μg per lane
Dilution : 1:2000

Anti-LAG3 antibody, Internal used in Western Blot (WB). GTX81605

GTX81605 WB Image

WB analysis of various samples using GTX81605 LAG3 antibody, Internal.
Lane 1: human ovary lysate
Lane 2: mouse liver lysate
Loading : 20 μg per lane
Dilution : 1:1000

Anti-LAG3 antibody, Internal used in Western Blot (WB). GTX81605

GTX81605 WB Image

WB analysis of mouse heart lysate using GTX81605 LAG3 antibody, Internal.
Loading : 20 μg per lane
Dilution : 1:2000

Anti-LAG3 antibody, Internal used in Flow cytometry (FACS). GTX81605

GTX81605 FACS Image

FACS analysis of Jurkat cells using GTX81605 LAG3 antibody, Internal.
Red : LAG3
Blue : Isotype control
Fixation : 2% paraformaldehyde (PFA) (10 min)
Permeabilization : 90% methanol (10 min)
Dilution : 1:25

Anti-LAG3 antibody, Internal used in Western Blot (WB). GTX81605

GTX81605 WB Image

WB analysis of 293T/17 whole cell lysate using GTX81605 LAG3 antibody, Internal.
Loading : 20 μg per lane
Dilution : 1:2000

Anti-LAG3 antibody, Internal used in Western Blot (WB). GTX81605

GTX81605 WB Image

WB analysis of various samples using GTX81605 LAG3 antibody, Internal.
Lane 1: 293T/17 whole cell lysate
Lane 2: mouse heart lysate
Loading : 20 μg per lane
Dilution : 1:2000

Anti-LAG3 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81605

GTX81605 IHC-P Image

IHC-P analysis of human thymus tissue using GTX81605 LAG3 antibody, Internal.
Antigen retrieval : heat-induced antigen retrieval with citrate buffer (pH6)
Dilution : 1:25

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399