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LAL antibody [N3C3]

KO/KD
Anti-LAL antibody [N3C3] used in Western Blot (WB). GTX101169
Anti-LAL antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101169
Anti-LAL antibody [N3C3] used in Western Blot (WB). GTX101169
Anti-LAL antibody [N3C3] used in Western Blot (WB). GTX101169
Anti-LAL antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101169
Anti-LAL antibody [N3C3] used in Western Blot (WB). GTX101169

Cat. No. GTX101169

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all LAL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Mouse liver , A549 , H1299 , HCT116

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LAL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617396

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lipase A, lysosomal acid type , CESD , LAL

Cellular Localization

Lysosome

Background

This gene encodes lipase A, the lysosomal acid lipase (also known as cholesterol ester hydrolase). This enzyme functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides. Mutations in this gene can result in Wolman disease and cholesteryl ester storage disease. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LAL antibody [N3C3] used in Western Blot (WB). GTX101169

GTX101169 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAL antibody [N3C3] (GTX101169) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LAL antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101169

GTX101169 ICC/IF Image

LAL antibody [N3C3] detects LAL protein at lysosome by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: LAL stained by LAL antibody [N3C3] (GTX101169) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-LAL antibody [N3C3] used in Western Blot (WB). GTX101169

GTX101169 WB Image

Sample (50 μg of whole cell lysate)
A: mouse Liver
10% SDS PAGE
GTX101169 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LAL antibody [N3C3] used in Western Blot (WB). GTX101169

GTX101169 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAL antibody [N3C3] (GTX101169) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LAL antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX101169

GTX101169 IHC-P Image

LAL antibody [N3C3] detects LAL protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
LAL stained by LAL antibody [N3C3] (GTX101169) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LAL antibody [N3C3] used in Western Blot (WB). GTX101169

GTX101169 WB Image

KO/KD

The data was published in the journal Elife in 2019.PMID: 30801249

REFERENCE

Application Reference

Hsu MC et al. Int J Biol Sci 2024; 20 (5) : 1563-1577 Apigenin targets fetuin-A to ameliorate obesity-induced insulin resistance.
Rivera-Serrano EE et al. Elife 2019; 8 Cellular entry and uncoating of naked and quasi-enveloped human hepatoviruses.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169