LAL antibody [N3C3] detects LAL protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
LAL stained by LAL antibody [N3C3] (GTX101169) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAL antibody [N3C3] (GTX101169) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (50 μg of whole cell lysate)
A: mouse Liver
10% SDS PAGE
GTX101169 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

LAL antibody [N3C3] detects LAL protein at lysosome by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: LAL stained by LAL antibody [N3C3] (GTX101169) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAL antibody [N3C3] (GTX101169) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Elife in 2019.PMID: 30801249