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LAMP1 antibody [GT25212]

Anti-LAMP1 antibody [GT25212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634336
Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336
Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336
Anti-LAMP1 antibody [GT25212] used in IHC (Paraffin sections) (IHC-P). GTX634336
Anti-LAMP1 antibody [GT25212] used in IHC (Paraffin sections) (IHC-P). GTX634336
Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336

Cat No. GTX634336

Host

Mouse

Clonality

Monoclonal

Clone Name

GT25212

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
PRODUCT

Summary

LAMP1 antibody detects lysosome-associated membrane protein 1 (LAMP-1, or CD107a), a transmembrane glycoprotein that generally migrates at ~120 kDa on western blot. LAMP1 and LAMP2 are major protein components of lysosomal membranes, though both proteins appear to have a more dynamic distribution that includes endosomes, autolysosomes, and multivesicular bodies. Nevertheless, LAMP1 is often used as an indicator of lysosomes in cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LAMP1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888439

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lysosomal associated membrane protein 1 , CD107a , LAMPA , LGP120

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Endosome membrane; Single-pass type I membrane protein , Lysosome membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LAMP1 antibody [GT25212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634336

GTX634336 ICC/IF Image

LAMP1 antibody [GT25212] detects LAMP1 protein at lysosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: LAMP1 stained by LAMP1 antibody [GT25212] (GTX634336) diluted at 1:2000.
Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (GTX112141) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336

GTX634336 WB Image

Wild-type (WT) and LAMP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336

GTX634336 WB Image

HeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-LAMP1 antibody [GT25212] used in IHC (Paraffin sections) (IHC-P). GTX634336

GTX634336 IHC-P Image

LAMP1 antibody [GT25212] detects LAMP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
LAMP1 stained by LAMP1 antibody [GT25212] (GTX634336) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LAMP1 antibody [GT25212] used in IHC (Paraffin sections) (IHC-P). GTX634336

GTX634336 IHC-P Image

LAMP1 antibody [GT25212] detects LAMP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
LAMP1 stained by LAMP1 antibody [GT25212] (GTX634336) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336

GTX634336 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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