LAMP2 antibody [N2C2], Internal

Cat. No. GTX111812

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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Datasheet
Document

View product citations for antibody GTX111812 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , 293T membrane extract

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LAMP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950660

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysosomal associated membrane protein 2 , CD107b , LAMP-2 , LAMPB , LGP-96 , LGP110

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Endosome membrane; Single-pass type I membrane protein , Lysosome membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq]

Database

Gene ID: 3920 LAMP2
UniProt: P13473 LAMP2

Research Area

DATA IMAGES

GTX111812 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DU-145 xenograft , using LAMP2(GTX111812) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111812 WB Image

293T whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP2 antibody [N2C2], Internal (GTX111812) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty