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LAMP2 antibody

KO/KD Orthogonal
Anti-LAMP2 antibody used in Western Blot (WB). GTX103214
Anti-LAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103214
Anti-LAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103214
Anti-LAMP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103214
Anti-LAMP2 antibody used in Western Blot (WB). GTX103214

Cat. No. GTX103214

Reference 9
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LAMP2 products
PRODUCT

Summary

LAMP2 antibody detects lysosome-associated membrane protein 2 (LAMP-2, or CD107b), a transmembrane glycoprotein that generally migrates at ~120 kDa on western blot. LAMP-2 has a similar distribution to LAMP-1 in endolysosomal and autophagic organelles. Moreover, like LAMP-1, LAMP-2 has been used to study lysosome biology.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LAMP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10615814

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysosomal associated membrane protein 2 , CD107b , LAMP-2 , LAMPB , LGP-96 , LGP110

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Endosome membrane; Single-pass type I membrane protein , Lysosome membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LAMP2 antibody used in Western Blot (WB). GTX103214

GTX103214 WB Image

KO/KD

Wild-type (WT) and LAMP2 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP2 antibody (GTX103214) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-LAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103214

GTX103214 IHC-P Image

LAMP2 antibody detects LAMP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
LAMP2 stained by LAMP2 antibody (GTX103214) diluted at 1:1000.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Anti-LAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX103214

GTX103214 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using LAMP2(GTX103214) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LAMP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103214

GTX103214 ICC/IF Image

LAMP2 antibody detects LAMP2 protein at lysosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: LAMP2 stained by LAMP2 antibody (GTX103214) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.

Anti-LAMP2 antibody used in Western Blot (WB). GTX103214

GTX103214 WB Image

Orthogonal

293T whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LAMP2 antibody (GTX103214) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Shi Y et al. J Biol Chem 2022; 298 (9) : 102341 O-GlcNAcylation stabilizes the autophagy-initiating kinase ULK1 by inhibiting chaperone-mediated autophagy upon HPV infection.
Application : WB
Chen JC et al. Biomolecules 2019; 9 (9) In Vitro and In Silico Mechanistic Insights into miR-21-5p-Mediated Topoisomerase Drug Resistance in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Izumi H et al. Sci Rep 2019; 9 (1) : 2236 Recycling endosomal CD133 functions as an inhibitor of autophagy at the pericentrosomal region.
Application : ICC/IF
Reactivity : Human
Jin S.P. et al. Bioscience Reports 2018; (Epub) Inhibition of N-glycosylation by tunicamycin attenuates cell-cell adhesion via impaired desmosome formation in normal human epidermal keratinocytes.
Application : ICC/IF
Reactivity : Human
Kuo WY et al. Sci Rep 2018; 8 (1) : 13735 Noninvasive assessment of characteristics of novel anti-HER2 antibodies by molecular imaging in a human gastric cancer xenograft-bearing mouse model.
Application : ICC/IF
Reactivity : Human
Show More +
Submit a Reference

REVIEW

LAMP2 antibody Cat. No. GTX103214

Rating
( Average 4.2 based on 5 users reviews)
Application
Western Blot(WB)
( Average 4 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4.3 based on 3 users reviews)
Anti-LAMP2 antibody used in Western Blot (WB). GTX103214
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Biomolecules in 2019.PMID: 31505885
Anti-LAMP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103214
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019.PMID: 30783186
Anti-LAMP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103214
Date : Anonymous submitted on 19-Aug-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HeLa cells
Blocking : 3% BSA in PBS, 20°C, 0.08Hr
Fixation : 3.6% formaldehyde
Permeabilization /Antigen retrieval : 1% NP-40
Primary Antibody : 1:100, 4°C, 16Hr
Anti-LAMP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103214
Date : Anonymous submitted on 29-Jul-2013
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : ARPE19 cells
Blocking : 3% BSA in PBS, 25°C, 0.17Hr
Fixation : PBS PFA 4%
Permeabilization /Antigen retrieval : 0.1% Triton in PBS
Primary Antibody : 1:100, 25°C, 1.5Hr
Figure Note : Red : Actin, labelled by phalloidin TRITC, Green : LAMP2(GTX103214), Blue : DNA, labelled by DAPI
Anti-LAMP2 antibody used in Western Blot (WB). GTX103214
Date : Anonymous submitted on 22-Apr-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : F79 cells,F123 cells
Amount used : 40μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Submit a Review
Application Reference
Shi Y et al. J Biol Chem 2022; 298 (9):102341 O-GlcNAcylation stabilizes the autophagy-initiating kinase ULK1 by inhibiting chaperone-mediated autophagy upon HPV infection.
Application : WB
Chen JC et al. Biomolecules 2019; 9 (9) In Vitro and In Silico Mechanistic Insights into miR-21-5p-Mediated Topoisomerase Drug Resistance in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Izumi H et al. Sci Rep 2019; 9 (1):2236 Recycling endosomal CD133 functions as an inhibitor of autophagy at the pericentrosomal region.
Application : ICC/IF
Reactivity : Human
Jin S.P. et al. Bioscience Reports 2018; (Epub) Inhibition of N-glycosylation by tunicamycin attenuates cell-cell adhesion via impaired desmosome formation in normal human epidermal keratinocytes.
Application : ICC/IF
Reactivity : Human
Kuo WY et al. Sci Rep 2018; 8 (1):13735 Noninvasive assessment of characteristics of novel anti-HER2 antibodies by molecular imaging in a human gastric cancer xenograft-bearing mouse model.
Application : ICC/IF
Reactivity : Human
Lai MC et al. PLoS One 2016; 11 (4):e0153627 Hypoxia Induces Autophagy through Translational Up-Regulation of Lysosomal Proteins in Human Colon Cancer Cells.
Application : WB
Reactivity : Human
Lai CY et al. J Immunol 2015; 195 (8):3912-21 identification of * as a novel inhibitor for psoriasis-like inflammation induced by tlr7-9.
Application : WB
Reactivity : Human
Hung TM et al. Am J Pathol 2015; (Epub) Increased Autophagy Markers Are Associated with Ductular Reaction during the Development of Cirrhosis.
Application : WB
Reactivity : Human
Liu TP et al. Anticancer Drugs 2015; 26 (2):139-47 S-Adenosyl-L-methionine-competitive inhibitors of the histone methyltransferase EZH2 induce autophagy and enhance drug sensitivity in cancer cells.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169