GeneTex
United States (US)

LAMP2 antibody

Orthogonal
GTX103214_43006_20181025_IHC-P_19011519_749.jpg
GTX103214_43006_20180117_ICC_IF_19011506_888.jpg
GTX103214_40275_20170713_WB_Fraction_19011506_426.jpg
GTX103214_40275_IHC_19011506_942.jpg

Cat No. GTX103214

Reference 6
3
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
See all LAMP2 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , 293T membrane extract , *F79 , *F123 , *ARPE19

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LAMP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD107b antibody, LAMPB antibody, LGP110 antibody, LAMP2 antibody, CD107 antigen-like family member B antibody, lysosome-associated membrane glycoprotein 2 antibody, lysosome-associated membrane protein 2 antibody, lysosomal-associated membrane protein 2 antibody

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Endosome membrane; Single-pass type I membrane protein , Lysosome membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103214_43006_20181025_IHC-P_19011519_749.jpg

GTX103214 IHC-P Image

LAMP2 antibody detects LAMP2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
LAMP2 stained by LAMP2 antibody (GTX103214) diluted at 1:1000.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
GTX103214_43006_20180117_ICC_IF_19011506_888.jpg

GTX103214 ICC/IF Image

LAMP2 antibody detects LAMP2 protein at lysosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: LAMP2 stained by LAMP2 antibody (GTX103214) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.
GTX103214_40275_20170713_WB_Fraction_19011506_426.jpg

GTX103214 WB Image

Orthogonal

293T whole cell and membrane extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP2 antibody (GTX103214) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX103214_40275_IHC_19011506_942.jpg

GTX103214 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using LAMP2(GTX103214) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
REFERENCE

Application Reference

Jin S.P. et al. Bioscience Reports 2018; (Epub ahead of print) Inhibition of N-glycosylation by tunicamycin attenuates cell-cell adhesion via impaired desmosome formation in normal human epidermal keratinocytes.
Application : ICC/IF
Reactivity : Human
Kuo WY et al. Sci Rep 2018; 8 (1) : 13735 Noninvasive assessment of characteristics of novel anti-HER2 antibodies by molecular imaging in a human gastric cancer xenograft-bearing mouse model.
Application : ICC/IF
Reactivity : Human
Lai MC et al. PLoS One 2016; 11 (4) : e0153627 Hypoxia Induces Autophagy through Translational Up-Regulation of Lysosomal Proteins in Human Colon Cancer Cells.
Application : WB
Reactivity : Human
Lai CY et al. J Immunol 2015; 195 (8) : 3912-21 Identification of Thiostrepton as a Novel Inhibitor for Psoriasis-like Inflammation Induced by TLR7-9.
Application : WB
Reactivity : Human
Hung TM et al. Am J Pathol 2015; (Epub ahead of print]) Increased Autophagy Markers Are Associated with Ductular Reaction during the Development of Cirrhosis.
Application : WB
Reactivity : Human
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REVIEW

LAMP2 antibody Cat No. GTX103214

Rating
( Average 3.7 based on 3 users reviews)
Application
Western Blot(WB)
( Average 3 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 19-Aug-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HeLa cells
Blocking : 3% BSA in PBS, 20°C, 0.08Hr
Fixation : 3.6% formaldehyde
Permeabilization /Antigen retrieval : 1% NP-40
Primary Antibody : 1:100, 4°C, overnightHr
Date : Anonymous submitted on 29-Jul-2013
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : ARPE19 cells
Blocking : 3% BSA in PBS, 25°C, 0.17Hr
Fixation : PBS PFA 4%
Permeabilization /Antigen retrieval : 0.1% Triton in PBS
Primary Antibody : 1:100, 25°C, 1.5Hr
Figure Note : Red : Actin, labelled by phalloidin TRITC, Green : LAMP2(GTX103214), Blue : DNA, labelled by DAPI
Date : Anonymous submitted on 22-Apr-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : F79 cells,F123 cells
Amount used : 40μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, overnightHr
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169