PRODUCT
Summary
LAMP2 antibody detects lysosome-associated membrane protein 2 (LAMP-2, or CD107b), a transmembrane glycoprotein that generally migrates at ~120 kDa on western blot. LAMP-2 has a similar distribution to LAMP-1 in endolysosomal and autophagic organelles. Moreover, like LAMP-1, LAMP-2 has been used to study lysosome biology.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , 293T membrane extract , *F79 , *F123 , *ARPE19
Predict Reactivity
Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.63 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human LAMP2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_10615814
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
lysosomal associated membrane protein 2 , CD107b , LAMP-2 , LAMPB , LGP-96 , LGP110
Cellular Localization
Cell membrane; Single-pass type I membrane protein , Endosome membrane; Single-pass type I membrane protein , Lysosome membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX103214 WB Image
Wild-type (WT) and LAMP2 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP2 antibody (GTX103214) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX103214 IHC-P Image
LAMP2 antibody detects LAMP2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.LAMP2 stained by LAMP2 antibody (GTX103214) diluted at 1:1000.Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min
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GTX103214 ICC/IF Image
LAMP2 antibody detects LAMP2 protein at lysosome by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: LAMP2 stained by LAMP2 antibody (GTX103214) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10μm.
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GTX103214 IHC-P Image
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using LAMP2(GTX103214) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX103214 WB Image
293T whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LAMP2 antibody (GTX103214) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
LAMP2 antibody
Cat No. GTX103214
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Rating
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( Average 4.2 based on 5 users reviews)
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Application
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Western Blot(WB)
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( Average 4 based on 2 users reviews)
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Immunocytochemistry/ Immunofluorescence(ICC/IF)
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( Average 4.3 based on 3 users reviews)
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Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Sci Rep in 2020.
PMID: 31980707
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
ICC/IF
Sample Species :
Hu
Figure Note :
The data was peer reviewed and published in the journal Cell Death Dis in 2014.
PMID: 25429617
Date :
Anonymous submitted on 19-Aug-2015
Application Tested :
ICC/IF
Sample Species :
Hu
Sample :
HeLa cells
Blocking :
3% BSA in PBS, 20°C, 0.08Hr
Fixation :
3.6% formaldehyde
Permeabilization /Antigen retrieval :
1% NP-40
Primary Antibody :
1:100, 4°C, 16Hr
Date :
Anonymous submitted on 29-Jul-2013
Application Tested :
ICC/IF
Sample Species :
Hu
Sample :
ARPE19 cells
Blocking :
3% BSA in PBS, 25°C, 0.17Hr
Fixation :
PBS PFA 4%
Permeabilization /Antigen retrieval :
0.1% Triton in PBS
Primary Antibody :
1:100, 25°C, 1.5Hr
Figure Note :
Red : Actin, labelled by phalloidin TRITC, Green : LAMP2(GTX103214), Blue : DNA, labelled by DAPI
Date :
Anonymous submitted on 22-Apr-2013
Application Tested :
WB
Sample Species :
Hu
Sample :
F79 cells,F123 cells
Amount used :
40μg
Blocking :
5% non-fat milk, 25°C, 1Hr
Primary Antibody :
1:2000, 4°C, 16Hr
