APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa (starvation and 200ng/ml EGF treatment for 15min) , HUVEC , 293T , A431 , HeLa , HepG2
Predict Reactivity
Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.48 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human LAMP2A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886700
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
lysosomal associated membrane protein 2 , CD107b , LAMP-2 , LAMPB , LGP-96 , LGP110
Cellular Localization
Cell membrane; Single-pass type I membrane protein , Endosome membrane; Single-pass type I membrane protein , Lysosome membrane; Single-pass type I membrane protein
Background
The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX132655 WB Image
Untreated (–) and treated (+) 293T whole cell and membrane extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Lamp2A antibody (GTX132655) diluted at 1:1000. (ME: membrane extract)
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GTX132655 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP2A antibody (GTX132655) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX132655 WB Image
Untreated (–) and treated (+) HeLa or HUVEC whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamp2A antibody (GTX132655) diluted at 1:1000.
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REFERENCE
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REVIEW
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