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LAMP3 antibody

Anti-LAMP3 antibody used in Western Blot (WB). GTX112715
Anti-LAMP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112715

Cat. No. GTX112715

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LAMP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LAMP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950661

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysosomal associated membrane protein 3 , CD208 , DC LAMP , DC-LAMP , DCLAMP , LAMP , LAMP-3 , TSC403

Cellular Localization

Lysosome membrane; Single-pass type I membrane protein

Background

Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al., 1998 [PubMed 9768752]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-LAMP3 antibody used in Western Blot (WB). GTX112715

GTX112715 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX112715 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LAMP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112715

GTX112715 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using LAMP3(GTX112715) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169