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LAMP3 antibody

Anti-LAMP3 antibody used in Western Blot (WB). GTX112715
Anti-LAMP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112715

Cat No. GTX112715

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all LAMP3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

HeLa , Molt-4

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LAMP3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950661

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

lysosomal associated membrane protein 3 , CD208 , DC LAMP , DC-LAMP , DCLAMP , LAMP , LAMP-3 , TSC403

Cellular Localization

Lysosome membrane; Single-pass type I membrane protein

Background

Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al., 1998 [PubMed 9768752]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-LAMP3 antibody used in Western Blot (WB). GTX112715

GTX112715 WB Image

Sample (30 μg of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX112715 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LAMP3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112715

GTX112715 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using LAMP3(GTX112715) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169