APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse liver
Predict Reactivity
Human, Zebrafish, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human LARGE. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885416
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
LARGE xylosyl- and glucuronyltransferase 1 , LARGE , MDC1D , MDDGA6 , MDDGB6
Cellular Localization
Golgi apparatus membrane; Single-pass type II membrane protein
Background
This gene, which is one of the largest in the human genome, encodes a member of the N-acetylglucosaminyltransferase gene family. It encodes a glycosyltransferase which participates in glycosylation of alpha-dystroglycan, and may carry out the synthesis of glycoprotein and glycosphingolipid sugar chains. It may also be involved in the addition of a repeated disaccharide unit. Mutations in this gene cause MDC1D, a novel form of congenital muscular dystrophy with severe mental retardation and abnormal glycosylation of alpha-dystroglycan. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX117180 IHC-P Image
LARGE antibody detects LARGE protein at cytosol on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
LARGE antibody (GTX117180) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX117180 IHC-P Image
LARGE antibody detects LARGE protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
LARGE antibody (GTX117180) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX117180 WB Image
LARGE antibody detects LARGE protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with LARGE antibody (GTX117180) at a dilution of 1:1000.
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REFERENCE
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REVIEW
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