Immunofluorescence analysis of methanol-fixed A431, using LARS2(GTX110083) antibody at 1:200 dilution.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LARS2 antibody [N2C1], Internal (GTX110083) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LARS2(GTX110083) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LARS2 antibody [N2C1], Internal (GTX110083) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

LARS2 antibody [N2C1], Internal detects LARS2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LARS2 protein stained by LARS2 antibody [N2C1], Internal (GTX110083) diluted at 1:500.
Blue: Hoechst 33342 staining.