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LARS2 antibody [N2C1], Internal

Anti-LARS2 antibody [N2C1], Internal used in Western Blot (WB). GTX110083
Anti-LARS2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110083
Anti-LARS2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110083
Anti-LARS2 antibody [N2C1], Internal used in Western Blot (WB). GTX110083
Anti-LARS2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX110083

Cat. No. GTX110083

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LARS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LARS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950663

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

leucyl-tRNA synthetase 2, mitochondrial , HLASA , LEURS , PRLTS4 , mtLeuRS

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a class 1 aminoacyl-tRNA synthetase, mitochondrial leucyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LARS2 antibody [N2C1], Internal used in Western Blot (WB). GTX110083

GTX110083 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LARS2 antibody [N2C1], Internal (GTX110083) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LARS2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110083

GTX110083 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using LARS2(GTX110083) antibody at 1:200 dilution.

Anti-LARS2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110083

GTX110083 ICC/IF Image

LARS2 antibody [N2C1], Internal detects LARS2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LARS2 protein stained by LARS2 antibody [N2C1], Internal (GTX110083) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-LARS2 antibody [N2C1], Internal used in Western Blot (WB). GTX110083

GTX110083 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LARS2 antibody [N2C1], Internal (GTX110083) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LARS2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX110083

GTX110083 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LARS2(GTX110083) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169