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LARS2 antibody [N2C1], Internal

Anti-LARS2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110083
Anti-LARS2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX110083
Anti-LARS2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110083
Anti-LARS2 antibody [N2C1], Internal used in Western Blot (WB). GTX110083
Anti-LARS2 antibody [N2C1], Internal used in Western Blot (WB). GTX110083

Cat No. GTX110083

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LARS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950663

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

leucyl-tRNA synthetase 2, mitochondrial , HLASA , LEURS , PRLTS4 , mtLeuRS

Cellular Localization

Mitochondrion matrix

Background

This gene encodes a class 1 aminoacyl-tRNA synthetase, mitochondrial leucyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LARS2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110083

GTX110083 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using LARS2(GTX110083) antibody at 1:200 dilution.

Anti-LARS2 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX110083

GTX110083 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LARS2(GTX110083) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-LARS2 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110083

GTX110083 ICC/IF Image

LARS2 antibody [N2C1], Internal detects LARS2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LARS2 protein stained by LARS2 antibody [N2C1], Internal (GTX110083) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-LARS2 antibody [N2C1], Internal used in Western Blot (WB). GTX110083

GTX110083 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with LARS2 antibody [N2C1], Internal (GTX110083) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LARS2 antibody [N2C1], Internal used in Western Blot (WB). GTX110083

GTX110083 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LARS2 antibody [N2C1], Internal (GTX110083) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for LARS2 antibody [N2C1], Internal (GTX110083). Be the first to share your publications with this product.

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf