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LASS2 antibody [HL1717]

Recombinant antibody KO/KD
Anti-LASS2 antibody [HL1717] used in Western Blot (WB). GTX637316
Anti-LASS2 antibody [HL1717] used in IHC (Paraffin sections) (IHC-P). GTX637316
Anti-LASS2 antibody [HL1717] used in IHC (Paraffin sections) (IHC-P). GTX637316
Anti-LASS2 antibody [HL1717] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637316
Anti-LASS2 antibody [HL1717] used in Western Blot (WB). GTX637316

Cat. No. GTX637316

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1717

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LASS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human LASS2. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ceramide synthase 2 , L3 , LASS2 , SP260 , TMSG1

Cellular Localization

Endoplasmic reticulum membrane

Background

This gene encodes a protein that has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein may play a role in the regulation of cell growth. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-LASS2 antibody [HL1717] used in Western Blot (WB). GTX637316

GTX637316 WB Image

KO/KD

Wild-type (WT) and CERS2 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LASS2 antibody [HL1717] (GTX637316) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-LASS2 antibody [HL1717] used in IHC (Paraffin sections) (IHC-P). GTX637316

GTX637316 IHC-P Image

LASS2 antibody [HL1717] detects LASS2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
LASS2 stained by LASS2 antibody [HL1717] (GTX637316) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LASS2 antibody [HL1717] used in IHC (Paraffin sections) (IHC-P). GTX637316

GTX637316 IHC-P Image

LASS2 antibody [HL1717] detects LASS2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
LASS2 stained by LASS2 antibody [HL1717] (GTX637316) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LASS2 antibody [HL1717] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637316

GTX637316 ICC/IF Image

LASS2 antibody [HL1717] detects LASS2 protein at endoplasmic reticulum and nuclear envelope by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: LASS2 stained by LASS2 antibody [HL1717] (GTX637316) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-LASS2 antibody [HL1717] used in Western Blot (WB). GTX637316

GTX637316 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LASS2 antibody [HL1717] (GTX637316) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations