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LASS2 antibody

KO/KD
Anti-LASS2 antibody used in Western Blot (WB). GTX130744
Anti-LASS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130744
Anti-LASS2 antibody used in Western Blot (WB). GTX130744
Anti-LASS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX130744

Cat. No. GTX130744

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all LASS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LASS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886337

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ceramide synthase 2 , L3 , LASS2 , SP260 , TMSG1

Cellular Localization

Nucleus membrane; Multi-pass membrane protein (Potential) , Endoplasmic reticulum membrane; Multi-pass membrane protein

Background

This gene encodes a protein that has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein may play a role in the regulation of cell growth. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LASS2 antibody used in Western Blot (WB). GTX130744

GTX130744 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LASS2 antibody (GTX130744) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LASS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130744

GTX130744 ICC/IF Image

LASS2 antibody detects LASS2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: LASS2 protein stained by LASS2 antibody (GTX130744) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-LASS2 antibody used in Western Blot (WB). GTX130744

GTX130744 WB Image

KO/KD

Wild-type (WT) and CERS2 knockout (KO) 293T WT cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LASS2 antibody (GTX130744) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LASS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX130744

GTX130744 IHC-P Image

LASS2 antibody detects LASS2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
LASS2 stained by LASS2 antibody (GTX130744) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169