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LAT antibody

Anti-LAT antibody used in Western Blot (WB). GTX104771
Anti-LAT antibody used in Western Blot (WB). GTX104771
Anti-LAT antibody used in Western Blot (WB). GTX104771
Anti-LAT antibody used in IHC (Paraffin sections) (IHC-P). GTX104771
Anti-LAT antibody used in IHC (Paraffin sections) (IHC-P). GTX104771

Cat No. GTX104771

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Jurkat , Jurkat membrane extract
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human LAT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

linker for activation of T cells,IMD52,LAT1,pp36

Cellular Localization

Cell membrane; Single-pass type III membrane protein

Background

The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-LAT antibody used in Western Blot (WB). GTX104771

GTX104771 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membranes were blotted with LAT antibody (GTX104771) diluted at 1:500 and competitor's antibody (sc-7948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-LAT antibody used in Western Blot (WB). GTX104771

GTX104771 WB Image

Jurkat whole cell and membrane extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with LAT antibody (GTX104771) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-LAT antibody used in Western Blot (WB). GTX104771

GTX104771 WB Image

Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membranes were blotted with LAT antibody (GTX104771) diluted at 1:1000 and competitor's antibody (sc-7948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-LAT antibody used in IHC (Paraffin sections) (IHC-P). GTX104771

GTX104771 IHC-P Image

LAT antibody detects LAT protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
LAT stained by LAT antibody (GTX104771) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LAT antibody used in IHC (Paraffin sections) (IHC-P). GTX104771

GTX104771 IHC-P Image

LAT antibody detects LAT protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
LAT stained by LAT antibody (GTX104771) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf