Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with LAT antibody (GTX104771) diluted at 1:500 and competitor's antibody (sc-7948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with LAT antibody (GTX104771) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with LAT antibody (GTX104771) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Jurkat whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with LAT antibody (GTX104771) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

LAT antibody detects LAT protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
LAT stained by LAT antibody (GTX104771) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

LAT antibody detects LAT protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
LAT stained by LAT antibody (GTX104771) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with LAT antibody (GTX104771) diluted at 1:1000 and competitor's antibody (sc-7948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.