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LAT antibody

Comparison Orthogonal
Anti-LAT antibody used in Western Blot (WB). GTX104771
Anti-LAT antibody used in Western Blot (WB). GTX104771
Anti-LAT antibody used in IHC (Paraffin sections) (IHC-P). GTX104771
Anti-LAT antibody used in IHC (Paraffin sections) (IHC-P). GTX104771
Anti-LAT antibody used in Western Blot (WB). GTX104771
Anti-LAT antibody used in Western Blot (WB). GTX104771
Anti-LAT antibody used in Western Blot (WB). GTX104771

Cat. No. GTX104771

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all LAT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Jurkat membrane extract

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human LAT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241020

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

linker for activation of T cells , IMD52 , LAT1 , pp36

Cellular Localization

Cell membrane; Single-pass type III membrane protein

Background

The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LAT antibody used in Western Blot (WB). GTX104771

GTX104771 WB Image

Comparison

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with LAT antibody (GTX104771) diluted at 1:500 and competitor's antibody (sc-7948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-LAT antibody used in Western Blot (WB). GTX104771

GTX104771 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with LAT antibody (GTX104771) diluted at 1:1000 and competitor's antibody (sc-7948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-LAT antibody used in IHC (Paraffin sections) (IHC-P). GTX104771

GTX104771 IHC-P Image

LAT antibody detects LAT protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
LAT stained by LAT antibody (GTX104771) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LAT antibody used in IHC (Paraffin sections) (IHC-P). GTX104771

GTX104771 IHC-P Image

LAT antibody detects LAT protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lymph node.
LAT stained by LAT antibody (GTX104771) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LAT antibody used in Western Blot (WB). GTX104771

GTX104771 WB Image

Orthogonal

Jurkat whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with LAT antibody (GTX104771) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-LAT antibody used in Western Blot (WB). GTX104771

GTX104771 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with LAT antibody (GTX104771) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LAT antibody used in Western Blot (WB). GTX104771

GTX104771 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with LAT antibody (GTX104771) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169