LAT1 / SLC7A5 antibody [HL2353]

Cat. No. GTX638556

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL2353
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all LAT1 / SLC7A5 products

Datasheet

View product citations for antibody GTX638556 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Product Note

This antibody was raised against human LAT1 / SLC7A5 Intracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human LAT1 / SLC7A5. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 7 member 5 , 4F2LC , CD98 , D16S469E , E16 , LAT1 , MPE16

Cellular Localization

Cytoplasm, cytosol

Background

Enables L-leucine transmembrane transporter activity; L-tryptophan transmembrane transporter activity; and thyroid hormone transmembrane transporter activity. Involved in carboxylic acid transport; thyroid hormone transport; and xenobiotic transport. Located in cytosol; intracellular membrane-bounded organelle; and plasma membrane. Is integral component of membrane. Part of amino acid transport complex; apical plasma membrane; and microvillus membrane. [provided by Alliance of Genome Resources, Apr 2022]

Database

Gene ID: 8140 SLC7A5
UniProt: Q01650 SLC7A5

Research Area

DATA IMAGES

GTX638556 WB Image

Non-transfected (–) and transfected (+) MCF-7 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638556 ICC/IF Image

LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX638556 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638556 IHC-P Image

LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX638556 WB Image

Boiled and unboiled HeLa whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

GTX638556 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638556 ICC/IF Image

LAT1 / SLC7A5 antibody [HL2353] detects LAT1 / SLC7A5 protein at cell membrane by immunofluorescent analysis.
Sample: Caco-2 cells were fixed in ice-cold MeOH for 5 min.
Green: LAT1 / SLC7A5 stained by LAT1 / SLC7A5 antibody [HL2353] (GTX638556) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

LAT1 / SLC7A5 antibody [HL2353]

Cat. No. GTX638556

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX638556 WB Image

GTX638556 ICC/IF Image

GTX638556 WB Image

GTX638556 IHC-P Image

GTX638556 WB Image

GTX638556 WB Image

GTX638556 ICC/IF Image

REFERENCE

REVIEW

Product

Research

Support

Company

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