GeneTex
United States (US)

LC3A/B antibody, N-term

GTX39751_4490_ICCIF_18121410_168.jpg
GTX39751_4492_WB_18121410_310.jpg

Cat No. GTX39751

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1/100-1/250
ICC/IF Assay dependent

Note :

WB
This antibody detects a band of approximately 14-15kDa corresponding to LC3-II, and a band of approximately 17kDa corresponding to LC3-I, in HeLa cell lysates.

Not tested in other applications.

Specificity/Sensitivity

This antibody recognizes both the LC3-I and LC3-II forms of MAP1LC3A and MAP1LC3B.
PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide sequence PSDRPFKQRRSFADC from the N-Terminal region of LC3A (NP_115903.1; NP_852610.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

4922501H04RIK Antibody , 1010001H21RIK Antibody , MAP1LC3A Antibody , LC3A Antibody

Background

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]
DATA IMAGES
GTX39751_4490_ICCIF_18121410_168.jpg

GTX39751 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX39751 LC3A/B antibody. Cell were fixed in 3% PFA and permeabilized with 0.3% Triton X-100.

GTX39751_4492_WB_18121410_310.jpg

GTX39751 WB Image

WB analysis of HeLa cell lysate using GTX39751 LC3A/B antibody, N-term.

Package List Price ($)
$ 229