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LC3A/B antibody, N-term

Anti-LC3A/B antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX39752
Anti-LC3A/B antibody, N-term used in Western Blot (WB). GTX39752

Cat. No. GTX39752

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μg ($619)
See all LC3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/100-1/250
ICC/IF Assay dependent

Note :

WB
This antibody detects a band of approximately 14-15kDa corresponding to LC3-II, and a band of approximately 17kDa corresponding to LC3-I, in HeLa cell lysates.
Not tested in other applications.

Product Note

This antibody recognizes both the LC3-I and LC3-II forms of MAP1LC3A and MAP1LC3B.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide sequence PSDRPFKQRRSFADC from the N-Terminal region of LC3A (NP_115903.1; NP_852610.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_11169240

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

4922501H04RIK Antibody , 1010001H21RIK Antibody , MAP1LC3A Antibody , LC3A Antibody

Background

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]

Research Area

DATA IMAGES

Anti-LC3A/B antibody, N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX39752

GTX39752 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX39752 LC3A/B antibody, N-term.

Anti-LC3A/B antibody, N-term used in Western Blot (WB). GTX39752

GTX39752 WB Image

WB analysis of HeLa cell lysate using GTX39752 LC3A/B antibody, N-term.

REFERENCE

Application Reference

Chang HP et al. Int J Oncol 2018; (Epub) pterostilbene modulates the suppression of multidrug resistance protein-1 and triggers autophagic and apoptotic mechanisms in *-resistant human oral cancer car cells via akt signaling.
Application : WB
Reactivity : Human
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 619