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LC3A antibody [166AT1234]

Anti-LC3A antibody [166AT1234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17380
Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380
Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380
Anti-LC3A antibody [166AT1234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17380

Cat No. GTX17380

Host

Mouse

Clonality

Monoclonal

Clone Name

166AT1234

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC

Reactivity

Human, Mouse, Rat
Package
200 μl ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:25-200
IHC-P Assay dependent
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.09% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This LC3A antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).

Purification

Protein G purified, followed by dialysis against PBS.

Conjugation

Unconjugated

RRID

AB_2887560

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-LC3A antibody [166AT1234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17380

GTX17380 ICC/IF Image

ICC/IF analysis of U251 cells treated with Chloroquine (50 μM,16h) using GTX17380 LC3A antibody [166AT1234].
Green : LC3A
Blue : cell nuclear
Fixation : 4% paraformaldehyde (PFA) (20 min)
Permeabilization : Triton X-100 (0.2%, 30 min)
Dilution : 1:200

Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

GTX17380 WB Image

WB analysis of multiple cell lysate using GTX17380 LC3A antibody [166AT1234].
Lane 1: Y79 (soluble fraction of cell extract)
Lane 2: 293 transfected with human LC3A (whole cell extract)
Dilution : 1:1000

Anti-LC3A antibody [166AT1234] used in Western Blot (WB). GTX17380

GTX17380 WB Image

WB analysis of various samples using GTX17380 LC3A antibody [166AT1234].
Lane 1: human brain lysates
Lane 2: mouse brain lysates
Lane 3: rat brain lysates
Dilution : 1:2000

Anti-LC3A antibody [166AT1234] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17380

GTX17380 ICC/IF Image

ICC/IF analysis of U251 cells untreatd (left) or treated with Chloroquine (50 μM,16h) (right) using GTX17380 LC3A antibody [166AT1234].
Green : LC3A
Blue : DAPI
Dilution : 1:25

REFERENCE

REVIEW

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Application Reference
Clone Reference
SDS
PBS.pdf
Sodium Azide.pdf