LC3A antibody [5G10]

Cat. No. GTX34067

Host	Mouse
Clonality	Monoclonal
Clone Name	5G10
Isotype	IgG1
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all LC3A products

Datasheet
Document

View product citations for antibody GTX34067 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:2000
IHC-P	1:100-1:200

Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic peptide derived from LC3A

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATG8E,LC3,LC3A,MAP1ALC3,MAP1BLC3,MAP1LC3A,microtubule associated protein 1 light chain 3 alpha

Cellular Localization

Cytoplasm,Cytoskeleton,Endomembrane system,Cytoplasmic vesicle,Autophagosome membrane,Autophagosome

Background

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]

Database

Gene ID: 84557 MAP1LC3A
UniProt: Q9H492 MAP1LC3A

Research Area

DATA IMAGES

GTX34067 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX34067 LC3A antibody [5G10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34067 WB Image

WB analysis of various sample lysates using GTX34067 LC3A antibody [5G10].
Lane 1 : HeLa cell lysate
Lane 2 : 3T3 cell lysate
Lane 3 : Rat brain tissue lysate

GTX34067 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX34067 LC3A antibody [5G10].
Dilution : 1:200

GTX34067 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX34067 LC3A antibody [5G10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34067 IHC-P Image

IHC-P analysis of human uterus cancer tissue using GTX34067 LC3A antibody [5G10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34067 IHC-P Image

IHC-P analysis of human uterus tissue using GTX34067 LC3A antibody [5G10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34067 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX34067 LC3A antibody [5G10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34067 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX34067 LC3A antibody [5G10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34067 IHC-P Image

IHC-P analysis of human stomach tissue using GTX34067 LC3A antibody [5G10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34067 IHC-P Image

IHC-P analysis of human heptacarcinoma tissue using GTX34067 LC3A antibody [5G10].
Dilution : 1:200

GTX34067 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34067 LC3A antibody [5G10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34067 IHC-P Image

IHC-P analysis of mouse colon tissue using GTX34067 LC3A antibody [5G10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34067 IHC-P Image

IHC-P analysis of human stomach cancer tissue using GTX34067 LC3A antibody [5G10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34067 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX34067 LC3A antibody [5G10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34067 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX34067 LC3A antibody [5G10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

GTX34067 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX34067 LC3A antibody [5G10]. Negative control (the lower left coner) was secondary antibody only.
Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98ºC, 20min)
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package	List Price ($)
100 μl	$ 399
Qty

LC3A antibody [5G10]

Cat. No. GTX34067

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX34067 IHC-P Image

GTX34067 WB Image

GTX34067 IHC-P Image

GTX34067 IHC-P Image

GTX34067 IHC-P Image

GTX34067 IHC-P Image

GTX34067 IHC-P Image

GTX34067 IHC-P Image

GTX34067 IHC-P Image

GTX34067 IHC-P Image

GTX34067 IHC-P Image

GTX34067 IHC-P Image

GTX34067 IHC-P Image

GTX34067 IHC-P Image

GTX34067 IHC-P Image

GTX34067 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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