GeneTex
United States (US)

LC3A antibody [5G10]

Anti-LC3A antibody [5G10] used in IHC (Paraffin sections) (IHC-P). GTX34067
Anti-LC3A antibody [5G10] used in Western Blot (WB). GTX34067
Anti-LC3A antibody [5G10] used in IHC (Paraffin sections) (IHC-P). GTX34067

Cat No. GTX34067

Host Mouse
Clonality Monoclonal
Clone Name 5G10
Isotype IgG1
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Specificity/Sensitivity

LC3A Mouse Monoclonal Antibody (5G10) detects endogenous levels of LC3A
PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic Peptide of LC3A

Purification

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Microtubule Associated Protein 1 Light Chain 3 Alpha,Atg8E,Lc3,Lc3A,Map1Alc3,Map1Blc3,Map1Lc3A

Background

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]

Database

Research Area

DATA IMAGES
Anti-LC3A antibody [5G10] used in IHC (Paraffin sections) (IHC-P). GTX34067

GTX34067 IHC-P Image

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Heptacarcinoma Tissue using LC3A Mouse Monoclonal Antibody diluted at 1:200.

Anti-LC3A antibody [5G10] used in Western Blot (WB). GTX34067

GTX34067 WB Image

Western Blot (WB) analysis of 1) HeLa Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue Lysate using LC3A Mouse Monoclonal Antibody diluted at 1:1000.

Anti-LC3A antibody [5G10] used in IHC (Paraffin sections) (IHC-P). GTX34067

GTX34067 IHC-P Image

Immunohistochemistry (IHC) analysis of paraffin-embedded Human Prostate Carcinoma Tissue using LC3A Mouse Monoclonal Antibody diluted at 1:200.

Package List Price ($)
$ 319